Development of an Immunochromatographic Kit for Rapid Diagnosis of H5 Avian Influenza Virus Infection
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概要
- 論文の詳細を見る
Highly pathogenic avian influenza (HPAI) caused by the H5N1 subtype has given rise to serious damage in poultry industries in Asia. The virus has expanded its geographical range to Europe and Africa, posing a great risk to human health as well. For the control of avian influenza, a rapid diagnosis by detecting the causative virus and identifying its subtype is essential. In the present study, a rapid diagnosis kit combining immunochromatography with enzyme immunoassay which detects the H5 HA antigen of influenza A virus was developed using newly established anti-H5 HA monoclonal antibodies. The present kit specifically detected all of the H5 influenza viruses tested, and did not react with the other HA subtypes. H5 HA antigens were detected from swabs and tissue homogenates of chickens infected with HPAI virus strain A/chicken/Yamaguchi/7/04 (H5N1) from 2 days post inoculation. The kit showed enough sensitivity and specificity for the rapid diagnosis of HPAI.
- Center for Academic Publications Japanの論文
- 2007-09-20
著者
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KIDA Hiroshi
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, H
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Kariwa Hiroaki
Hokkaido Univ. Sapporo Jpn
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SAKODA Yoshihiro
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, H
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Sakoda Yoshihiro
Hokkaido Univ. Sapporo Jpn
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Sakoda Yoshihiro
Laboratory Of Microbiology Department Of Disease Control Graduate School Of Veterinary Medicine Hokk
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TSUDA Yoshimi
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, H
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SAKABE Saori
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, H
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MOCHIZUKI Tsuyoshi
BL Corporation Limited
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NAMBA Yasuharu
BL Corporation Limited
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Sakabe Saori
Laboratory Of Microbiology Department Of Disease Control Graduate School Of Veterinary Medicine Hokk
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Kida Hiroshi
北海道大学大学院獣医学研究科微生物学教室
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Kida Hiroshi
Laboratory Of Microbiology Department Of Disease Control Hokkaido University Graduate School Of Vete
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Kida Hiroshi
Laboratory Of Microbiology Department Of Disease Control Graduate School Of Veterinary Medicine Hokk
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