<基礎的研究発生病理>9月齢SHRSP血小板の細胞内遊離カルシウム濃度変化, ATP分泌量, 凝集反応について

概要

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It has been reported that platelet releasing and aggregation activities are dependent largely on the cytosolic free calcium level. The responses of SHRSP platelets to platelet aggregation reagents decreased upon stimulation, compared with WKY platelets. With the platelets of 9-month-old SHRSP and WKY, we measured the reagent induced aggregation activities using fura-2AM and luciferin-luciferase. In the absence of extracellular Ca^<++>, each reagent, such as thrombin, collagen, ADP, ionomycin and TPA, elevated free calcium level to the same extent in samples of both SHRSP and WKY. With regard to ATP release, thrombin and collagen potentiated the action less in SHRSP platelets than in WKY, and the reagent induced ATP release was not affected by extracellular Ca^<-+>. Collagen and ADP induced aggregations showed lower activity in SHRSP than in WKY. TPA caused higher aggregation activity in SHRSP platelets than in WKY, in the presence of extracellular Ca^<++>. These results will indicate that calcium release must be followed by ATP release, and ATP release may be potentiated less while thrombin and TPA induced aggregation would be stimulated in SHRSP platelets compared with WKY, because C-kinase activity appears to be higher in SHRSP platelets than in WKY. The fibrinogen induced aggregation pathway on the plasma membrane in which ADP is involved, might be somewhat impaired in SHRSP platelets.
近畿大学の論文
1988-12-30