Analysis of Tissue-Specific Expression of Human Type II Collagen cDNA Driven by Different Sizes of the Upstream Region of the β-Casein Promoter
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概要
- 論文の詳細を見る
To investigate the ability of 1.8 kb or 3.1 kb bovine β-casein promoter sequences for the expression regulation of transgene in vivo, transgenic mice were produced with human type II collagen gene fused to 1.8 kb and 3.1 kb of bovine β-casein promoter by DNA microinjection. Five and three transgenic founder mice were produced using transgene constructs with 1.8 kb and 3.1 kb of bovine β-casein promoters respectively. Founder mice were outbred with the wild type to produce F1 and F2 progenies. Total RNAs were extracted from four tissues (mammary gland, liver, kidney, and muscle) of female F1 transgenic mice of each transgenic line following parturition. RT-PCR and Northern blot analysis revealed that the expression level of transgene was variable among the transgenic lines, but transgenic mice containing 1.8 kb of promoter sequences exhibited more leaky expression of transgene in other tissues compared to those with 3.1 kb promoter. Moreover, Western blot analysis of transgenic mouse milk showed that human type II collagen proteins secreted into the milk of lactating transgenic mice contained 1.8 kb and 3.1 kb of bovine β-casein promoter. These results suggest that promoter sequences of 3.1 kb bovine β-casein gene can be used for induction of mammary gland-specific expression of transgenes in transgenic animals.
- 社団法人 日本農芸化学会の論文
- 2006-01-23
著者
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Choi Yun
Department of Internal Medicine, Seoul National University College of Medicine, Cardiovascular Labor
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LEE Hong
Department of Family Medicine, School of Medicine, Ewha Womans University
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Kim Sun
Department Of Chemical Engineering Sogang University
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Choi Yun
Department Of Anatomy Chonbuk National University Medical School:research Center For Allergic Immune
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Yoo Seung
Division Of Biotechnology And Genetic Engineering Korea University
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Jin Dong
Research Center For Transgenic And Cloned Pigs Chungnam National University
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Naruse Kenji
Research Center For Transgenic And Cloned Pigs Chungnam National University
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Kim Sun
Department Of Applied Chemistry Faculty Of Engineering Osaka University
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Lee Hong
Department Of Ceramic Engineering Yonsei University
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Lee Hong
Department Of Food Science And Nutrition Daejin University
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Choi Yun
Department Of Animal Science And Technology Seoul National University
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Kim Sun
Department Of Animal Science And Technology Seoul National University
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Lee Hong
Department Of Bio-resources And Pnu-special Animal Biotechnology Center College Of Natural Resources & Life Science Pusan National University
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