Proteomic Analysis in Cyclosporin A-Induced Overgrowth of Human Gingival Fibroblasts

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概要

• 論文の詳細を見る

• Cyclosporin A (CsA) has been used as an immunosuppressive drug to prevent organ transplant rejection and to treat autoimmune diseases. CsA has a proliferative effect on human gingival fibroblasts (HGF) in vitro. However, the molecular mechanisms underlying CsA-induced proliferation in HGF remain to be elucidated. This study was aimed to investigate the CsA responsive proteins in HGF using systematic proteomic approach. Cell viability was determined by MTT assay and reactive oxygen species (ROS) was measured by fluorescent spectrometer. Proteins profiled by two-dimensional gel electrophoresis (2-DE) were identified by matrix-assisted laser desorption ionization time-of flight (MALDI-TOF) and electrospray ionization quadrupole time-of-flight mass spectrometry (EIQ-TOF MS). To confirm the expression changes of proteins by proteomics analysis, Western blot was performed using specific antibody. CsA increased the cell viability of HGF in a dose- and time-dependent manner. Significantly, seventeen proteins were overexpressed in the CsA-treated HGF, whereas three proteins were found to be expressed less than the untreated cells. The identified proteins were mainly related with cell proliferation, metabolism, and oxidation. The overexpression of peroxiredoxin 1 (Prx 1) confirmed by Western blotting and reduction of cytosolic reactive oxygen species (ROS) levels in the CsA-treated HGF demonstrated that Prx 1 may play a crucial role in the HGF proliferation induced by CsA. Upregulation of Galectin 3 in CsA-treated HGF indicated that it is related to CsA-induced proliferation. These proteomic analysis data will provide an efficient approach in understanding the mechanisms of HGF proliferation by CsA.

• 日本薬学会の論文

著者

• Kim Sun

  Department Of Chemical Engineering Sogang University

• Kim Won

  Department Of Endocrinology & Metabolism University Of Ulsan College Of Medicine

• JUNG Ji

  Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The Universit

• Kim Won

  Deparment Of Obstetrics & Gynecology College Of Medicine Pusan National University

• KWAK Yong

  Department of Pharmacology, Chonbuk National University Medical School

• Jung Ji

  Department Of Genetic Epidemiology Snp Genetics Inc.

• Kang Gi

  Department of Oral Physiology, Dental Science Research Institute, 2nd Stage of Brain Korea 21 for Sc

• Jeong Yeon

  Department of Oral Physiology, Dental Science Research Institute, 2nd Stage of Brain Korea 21 for Sc

• Kim Sun

  Department Of Applied Chemistry Faculty Of Engineering Osaka University

• Kwak Yong

  Department Of Neurology Hyoja Geriatric Hospital

• Kim Sun

  Department Of Animal Science And Technology Seoul National University

• Jeong Yeon

  Department Of Inorganic Materials Engineering Kyungpook National University

• Jeong Yeon

  Department Of Image System And Engineering Pukyong National University

• JUNG Ji

  Department of Anatomy, College of Medicine, Hallym University

• Kang Gi

  Department of Oral Physiology, Dental Science Research Institute, 2nd Stage of Brain Korea 21 for School of Dentistry, Chonnam National University School of Dentistry

• Kim Sun

  Department of Oral Physiology, Dental Science Research Institute, 2nd Stage of Brain Korea 21 for School of Dentistry, Chonnam National University School of Dentistry

• Jeong Yeon

  Department of Oral Physiology, Dental Science Research Institute, 2nd Stage of Brain Korea 21 for School of Dentistry, Chonnam National University School of Dentistry

• Jung Ji

  Department of Oral Physiology, Dental Science Research Institute, 2nd Stage of Brain Korea 21 for School of Dentistry, Chonnam National University School of Dentistry

• Kwak Yong

  Department of Pharmacology, Research Institute of Clinical Medicine, Chonbuk National University Medical School

• Kim Won

  Department of Oral Physiology, Dental Science Research Institute, 2nd Stage of Brain Korea 21 for School of Dentistry, Chonnam National University School of Dentistry

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