Bacterial Expression and Functional Characterization of a Rat Thyroid Hormone Sulfotransferase, ST1B1

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概要

• 論文の詳細を見る

• At least three forms of phenol sulfotransferase (ST) ST1B1, ST1A1 and ST1C1 are contained in rat livers. To identify the form contributing to the metabolism of 3, 3′, 5-triiodothyronine (T3), functional characterization of these forms was performed by expression in Escherichia coli. ST1B1 and ST1C1 were shown to be active on sulfation towards T3 with high affinity (Km: 44.4 and 25.8 μM, respectively), whereas ST1A1 had low affinity. In Western blotting using antibodies raised against the individual ST, hepatic contents of each ST were quantitatively determined. ST1B1 showed no clear sex-difference, whereas the level of ST1C1 was higher in adult males than adult females. The content of ST1B1 was 1.4, 6.8 and 10 times higher than that of ST1C1 in adult males, adult females and both sexes of immature rats, respectively. The developmental pattern of ST1B1 was similar to that of ST1A1, but differed from that of ST1C1. These results indicate that ST1B1 and ST1C1 are involved in T3 metabolism in rats and ST1B1 is the constitutive form across sexes and ages.

• 社団法人 日本薬理学会の論文
• 1999-04-01

著者

• Nagata Kiyoshi

  東北大学 薬学研究科薬物動態学

• Nagata Kiyoshi

  Division Of Drug Metabolism And Molecular Toxicology Graduate School Of Pharmaceutical Sciences Toho

• YAMAZOE Yasushi

  Division of Drug Metabolism and Molecular Toxicology, Graduate School of Pharmaceutical Sciences, To

• Yamazoe Y

  Division Of Drug Metabolism And Molecular Toxicology Graduate School Of Pharmaceutical Sciences Toho

• Yamazoe Yasushi

  Department Of Pharmacology School Of Medicine Keio University:department Of Analytical Biochemistry

• Nagata Kiyoshi

  九州大学 薬学研究院

• Fujita K

  Tohoku Univ. Sendai Jpn

• Shimada Miki

  Division of Drug Metabolism and Molecular Toxicology, Graduate School of Pharmaceutical Sciences, To

• FUJITA Ken-ichi

  Division of Drug Metabolism and Molecular Toxicology, Faculty of Pharmaceutical Sciences, Tohoku Uni

• Shimada M

  Division Of Drug Metabolism And Molecular Toxicology Graduate School Of Pharmaceutical Sciences Toho

• WATANABE Eriko

  Division of Drug Metabolism and Molecular Toxicology, Graduate School of Pharmaceutical Sciences, To

• Watanabe Etsushi

  Department Of Biopharmaceutics Faculty Of Pharmaceutical Sciences Science University Of Tokyo

• Shimada Miki

  Department Of Pharmacology School Of Medicine Keio University

• Fujita Ken-ichi

  Division Of Drug Metabolism And Molecular Toxicology Faculty Of Pharmaceutical Sciences Tohoku Unive

• Yamazoe Yasushi

  Division Of Drug Metabolism & Molecular Toxicology Graduate School Of Pharmaceutical Sciences To

• Watanabe Eriko

  Department Of Biopharmaceutics Faculty Of Pharmaceutical Sciences Science University Of Tokyo

• Yamazoe Yasushi

  Division Of Drug Metabolism And Molecular Toxicology Graduate School Of Pharmaceutical Sciences Toho

• Fujita Ken-ichi

  Department Of Biology And Geosciences Graduate School Of Science Osaka City University

• Nagata K

  Department Of Environmental And Health Science Tohoku Pharmaceutical University

• Shimada Mitsuyo

  Department Of Physiology Teikyo University School Of Medicine

• Shimada Miki

  Division of Drug Metabolism and Molecular Toxicology, Faculty of Pharmaceutical Sciences, Tohoku University

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