Antifungal Activity of Radicicol against Mucor flavus IFO 9560
スポンサーリンク
概要
- 論文の詳細を見る
The antifungal activity of radicicol against Mucor flavus IFO 9560 was investigated. Radicicol induced bursting of spores during germination and morphological changes of the mycelial tip such as overbranching and swelling during exponential growth. In addition, radicicol showed a dose-dependent inhibitory effect on spore germination. Radicicol also inhibited the incorporation of radioactive precursors into DNA, RNA, protein, and chitin fractions by 20-30%, but not into the lipid fraction. There were no inhibitory effects on either endogenous or exogenous cellular respiration. Moreover, leakage of UV-absorbing, phenol sulfate-positive, or folin reagent-positive materials from the mycelia was not observed at an early stage of growth inhibition. On the other hand, kinetic studies of chitin synthase in the untreated mycelia revealed that radicicol noncompetitively inhibited the enzyme at K_i of 87 μM. Furthermore, upon incubation of the normal mycelia with radicicol in 50 mM KH_2PO_4-NaOH buffer (pH 6.5) containing 10 mM MgCl_2, chitin synthase from the mycelia was inactivated gradually at first, and completely after 24-h incubation. These results suggested that radicicol exhibits the antifungal activity by disturbing cell wall biosynthesis through the inactivation of chitin synthase. However, at an early stage of growth inhibition, radicicol was thought to affect cellular function including nucleic acid and protein syntheses, in addition to the reversible noncompetitive inhibition of chitin synthase.
- 社団法人日本生物工学会の論文
- 1999-10-25
著者
-
Taniguchi M
Graduate School Of Science Osaka City University
-
TANIGUCHI Makoto
Department of Neurosurgery, Tokyo Metropolitan Neurological Hospital
-
Fujita K
Graduate School Of Science Osaka City University
-
Fujita K
Tohoku Univ. Sendai Jpn
-
FUJITA Ken-ichi
Department of Medical Oncology, Comprehensive Cancer Center, Saitama Medical University
-
Ping XU
Department of Mathematics, Pennsylvania State University
-
Taniguchi Makoto
Graduate School of Science, Osaka City University
-
IRIE MOTOKO
Department of Bio- and Geoscience, Graduate School of Science, Osaka City University
-
Taniguchi Makoto
Graduate School Of Science Osaka City University
-
Ping Xu
Department Of Biology Graduate School Of Science Osaka City University
-
Irie Motoko
Department Of Bio- And Geoscience Graduate School Of Science Osaka City University
-
Fujita Ken-ichi
Department Of Biology And Geosciences Graduate School Of Science Osaka City University
-
Taniguchi Makoto
Deprtment Of Bio- And Geoscience Graduate School Of Science Osaka City University
-
Taniguchi M
Taiyo Kagaku Co. Ltd. Mie Jpn
-
Taniguchi Makoto
Department Of Biology Faculty Of Science Osaka City University
-
Taniguchi Makoto
Department Of Bio- And Geoscience Gradaute School Of Science Osaka City University
-
TANIGUCHI Makoto
Department Medical Research Institute Kanazawa Medical University
関連論文
- Comparative Analysis of Spinal Hemangioblastomas in Sporadic Disease and Von Hippel-Lindau Syndrome
- Molecular Cloning and Characterization of Rat ST1B1 and Human ST1B2 cDNAs, Encoding Thyroid Hormone Sulfotransferases
- Association of ATP-Binding Cassette, Sub-family C, Number 2 (ABCC2) Genotype with Pharmacokinetics of Irinotecan in Japanese Patients with Metastatic Colorectal Cancer Treated with Irinotecan Plus Infusional 5-Fluorouracil/Leucovorin (FOLFIRI)(Miscellaneo
- CYP2A6 and the plasma level of 5-chloro-2,4-dihydroxypyridine are determinants of the pharmacokinetic variability of tegafur and 5-fluorouracil, respectively, in Japanese patients with cancer given S-1
- Enzymatic Characterization of Human Cytosolic Sulfotransferases : Identification of ST1B2 as a Thyroid Hormone Sulfotransferase
- Bacterial Expression and Functional Characterization of a Rat Thyroid Hormone Sulfotransferase, ST1B1
- Molecular Cloning, Expression, and Enzymatic Characterization of Rabbit Hydroxysteroid Sulfotransferase AST-RB2 (ST2A8)^1
- Molecular Cloning and Expression of an Amine Sulfotransferase cDNA: A New Gene Family of Cytosolic Sulfotransferases in Mammals^1
- 2B-08 Establishment of Genetically Engineered E.coli Highly Sensitive to Nitrosamines by Introducing Human CYP2E1
- Dynamical r-Matrices Coupled with Poisson Manifolds
- Preparation of Di-altro-β-cyclodextrins with Two Altrosides as the Macrocyclic Units through Selective Synthesis of Regioisomers of Di(2-O-mesitylenesulfonyl)-β-cyclodextrin
- Irreversible Deacylation of Plasma Membrane Phospholipids by the Combined Action of Mg^ and a Long-Chain Acyl-CoA Synthetase Inhibitor in Saccharomyces cerevisiae
- Isolation of an Acremonium sp. Capable of Liquefying Cross-Linked Poly(γ-Glutamic Acid) Hydrogels and the Fungal Enzyme Involved in the Disruption of γ-Ray Irradiation-Mediated Cross-Linking(MISCELLANEOUS)
- Flocculating Activity of Cross-linked Poly-γ-Glutamic Acid against Bentonite and Escherichia coli Suspension Pretreated with FeCl_3 and Its Interaction with Fe^(ENVIRONMENTAL BIOTECHNOLOGY)
- UK-2A, B, C and D, Novel Antifungal Antibiotics from Streptomyces sp. 517-02 V. Inhibition Mechanism of Bovine Heart Mitochondrial Cytochrome bc_1 by the Novel Antibiotic UK-2A
- UK-2A, B, C and D, Novel Antifungal Antibiotics from Streptomyces sp. 517-02 IV. Comparative Studies of UK-2A with Antimycin A_3 on Cytotoxic Activity and Reactive Oxygen Species Generation in LLC-PK1 Cells
- Establishment of Salmonella Tester Strains Co-expressing Cytochrome P450 and NADPH-cytochrome P450 Reductase.
- Application of Genetically Engineered Bacteria Carrying Human Cytochrome P450 to Toxicological Studies
- Establishment Of A Salmonella Tester Strain Ultra-Sensitive To Mutagenic Heterocyclic Amines
- DEVELOPMENT OF PRACTICAL METHODS WHICH MIMIC DRUG METABOLISM IN HUMANS.
- EXPRESSION OF RECOMBINANT HUMAN CYTOCHROME P450 (P450 OR CYP) 2A6 IN ESCHERICHIA COLI.
- "S2-3 STRATEGIC PROPOSALS TO AVOID TOXIC DRUG INTERACTIONS DURING NEW DRUG DEVELOPMENT : MOLECULAR TOXICOLOGY PROPOSALS (Strategy how we can learn drug-drug interaction during the development of a new drug)
- Pharmacogenetic impact of polymorphisms in the coding region of the UGT1A1 gene on SN-38 glucuronidation in Japanese patients with cancer
- Novel Single Nucleotide Polymorphism of UGT1A9 Gene in Japanese
- Novel Single Nucleotide Polymorphism of UGT1A7 Gene in Japanese
- Intramedullary Spinal Cord Abscess Treated With Antibiotic Therapy : Case Report and Review
- Identification of Phoslactomycin E as a Metabolite Inducing Hyphal Morphological Abnormalities in Aspergillus fumigatus IFO 5840
- Amplification of Vacuole-targeting Fungicidal Activity of Antibacterial Antibiotic Polymyxin B by Allicin, an Allyl Sulfur Compound from Garlic
- Synergistic Fungicidal Activities of Amphotericin B and N-Methyl-N"-dodecylguanidine : A Constituent of Polyol Macrolide Antibiotic Niphimycin
- Inhibitory Activity of 1-Farnesylpyridinium on the Spatial Control over the Assembly of Cell Wall Polysaccharides in Schizosaccharomyces pombe
- P-502 Structure-Activity Relationship Studies on UK-2A, A Novel Antifungal Antibiotic from Streptomyces sp. 517-02
- P-368 Identification of Phoslactomycin E as a Metabolite Inducing Hyphal Morphological Abnormalities of Aspergillus fumigatus IFO 5840
- Involvement of Oxidative Stress Induction in Na^+ Toxicity and Its Relation to the Inhibition of a Ca^-Dependent but Calcineurin-Independent Mechanism in Saccharomyces cerevisiae(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Biosynthesis of Poly-γ-Glutamic Acid in Plants : Transient Expression of Poly-γ-Glutamate Synthetase Complex in Tobacco Leaves(PLANT BIOTECHNOLOGY)
- Proposals for Wastewater Treatment by Applying Flocculating Activity of Cross-linked Poly-γ-Glutamic Acid(Environmental Biotechnology)
- Physicochemical Properties of Cross-linked Poly-γ-Glutamic Acid and Its Flocculating Activity against Kaolin Suspension (Environmental Biotechnology)
- Oxidative Stress-Dependent Inhibition of Yeast Cell Growth by Farnesylamine and Its Possible Relation to Amine Oxidase in the Mitochondrial Fraction(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- UK-2A, B, C and D, Novel Antifungal Antibiotics from Streptomyces sp. 517-02 : VI (3). Role of Substituents on Dilactone Ring of UK-2A and Antimycin A_3 against Generation of Reactive Oxygen Species in Porcine Renal Proximal Tubule LLC-PK_1 Cells
- Growth Inhibition Dependent on Reactive Oxygen Species Generated by C9-UK-2A, a Derivative of the Antifungal Antibiotic UK-2A, in Saccharomyces cerevisiae
- UK-2A,B,C, and D, Novel Antifungal Antibiotics from Streptomyces sp. 517-02 VIII. Reactive Oxygen Species Generated by C9-UK-2A, a Derivative of UK-2A, in Rhodotorula mucilaginosa IFO 0001
- UK-2A, B, C and D, Novel Antifungal Antibiotics from Streptomyces sp. 517-02 VI.(2). Structure-activity Relationships of UK-2A
- UK-2A, B, C, and D, Novel Antifungal Antibiotics from Streptomyces sp. 517-02 : VII. Membrane Injury Induced by C9-UK-2A, a Derivative of UK-2A, in Rhodotorula mucilaginosa IFO 0001
- L-2,5-Dihydrophenylalanine, an Inducer of Cathepsin-dependent Apoptosis in Human Promyelocytic Leukemia Cells(HL-60)
- Antifungal Activity of Radicicol against Mucor flavus IFO 9560
- Synergistic Combination of Direct Plasma Membrane Damage and Oxidative Stress as a Cause of Antifungal Activity of Polyol Macrolide Antibiotic Niphimycin
- Depletion of Glutathione as a Cause of the Promotive Effects of Polygodial, a Sesquiterpene on the Production of Reactive Oxygen Species in Saccharomyces cerevisiae
- The Mode of Action of AKD-2C, an Antifungal Antibiotic from Streptomyces sp. OCU-42815
- Synthesis of Uridine 5'-Monophosphate Glucose as an Inhibitor of UDP-Glucose Pyrophosphorylase
- Purification and Characterization of Poly(γ-glutamic acid) Hydrolase from a Filamentous Fungus, Myrothecium sp. TM-4222
- Screening for Microorganisms Having Poly(γ-glutamic acid) Endohydrolase Activity and the Enzyme Production by Myrothecium sp. TM-4222
- The Dependence of Catalytic Activities of Secondary Functional β-Cyclodextrins on Cavity Structures
- Clinical Characteristics and Surgical Management for Juxtafacet Cysts of the Lumbar Spine
- Hydrogen and Methane Fermentation of Rice Straw and Kitchen Leftovers
- Inhibitory Effects of Citrus Fruits on Cytochrome P450 3A (CYP3A) Activity in Humans(Miscellaneous)
- Informed Consent in Relation to Management of Acoustic Neurinoma : Clinical Analysis of 7-Year Results in 214 Consecutive Cases
- Endothelin ET_B Receptor-Mediated Action on Systemic and Renal Hemodynamics and Urine Formation in Deoxycorticosterone Acetate-Salt-Induced Hypertensive Rats
- UK-2A, B, C and D, Novel Antifungal Antibiotics from Streptomyces sp. 517-02 VI(1). Structure-Activity Relationship of UK-2A
- Synthesis of Diaryl Diselenides Having Chiral Pyrrolidine Rings with C_2 Symmetry. Their Application to the Asymmetric Methoxyselenenylation of trans-β-Methylstyrenes
- Asymmetric Oxyselenenylation of Olefins Using Optically Active Selenobinaphthyls and d-Menthol as a Nucleophile
- Synthesis of 1-Monoglycerides Having C_ Branched Chain Fatty Acids and Their Hemolysis Effects
- Radical Polymerization of Methyl Methacrylate by Captodative Substituted Morpholino Succinonitrile
- Purification and Characterization of Geranylgeranyl Diphosphate Synthase from Methanobacterium thermoformicicum SF-4
- Some Properties of F_-Reducing Hydrogenase of Methanobacterium thermoformicicum Strain SF-4
- Purification and Some Properties of Aspartate Aminotransferase of Methanobacterium thermoformicicum SF-4(Biological Chemistry)
- Novel Early-late Transition Metal Complexes. The First Thiolato Bridged Titanium-Ruthenium Complexes
- Chrysodin, an Antifungal Antimetabolite(Biological Chemistry)
- Polygodial-induced Sensitivity to Rifampicin and Actinomycin D of Saccharomyces cerevisiae(Biological Chemistry)
- Protective Effects of Ca^ on Cell Membrane Damage by Polygodial in Saccharomyces cerevisiae(Biological Chemistry)
- Potentiation by Polygodial of the Respiration Inhibiting Activity of Maesanin in Candida utilis(Biological Chemistry)
- Mode of Action of Polygodial, an Antifungal Sesquiterpene Dialdehyde(Biological Chemistry)
- Non-Radiochemical 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase Assay by Reversed-Phase HPLC without Using Ion-Pair Reagent
- UK-2A, B, C and D, Novel Antifungal Antibiotics from Streptomyces sp. 517-02 III. Absolute Configuration of an Antifungal Antibiotic, UK-2A, and Consideration of Its Conformation
- UK-1, a Novel Cytotoxic Metabolite from Streptomyces sp. 517-02 IV. Antifungal Action of Methyl UK-1
- UK-3A, a Novel Antifungal Antibiotic from Streptomyces sp. 517-02: Fermentation, Isolation, Structural Elucidation and Biological Properties
- UK-2A, B, C and D, Novel Antifungal Antibiotics from Streptomyces sp. 517-02 II. Structural Elucidation
- Mode of Action of Anhydrofulvic Acid against Candida utilis ATCC 42402 under Acidic Condition
- Methane Production from Hydrogen and Carbon Dioxide and Monoxide in a Column Bioreactor of Thermophilic Methanogens by Gas Recirculation
- Microbial Synthesis of 5-Fluorouridine Monophosphate and 5-Fluoro-2'-Deoxyuridine Monophosphate
- Effect of Steam Explosion Treatment on Enzymatic Hydrolysis of Palm Cake and Fiber as Solid Wastes and Natural Resources
- Studies on Cellular Response of Escherichia coli to UDP-Galactose Accumulation
- Production of Poly(γ-glutamic acid) by Bacillus subtilis F-2-01
- The Mode of Action of UK-2A and UK-3A, Novel Antifungal Antibiotics from Streptomyces sp. 517-02
- Mode of the Antifungal Action of Chrysodin in Candida albicans(Biological Chemistry)
- Citrinin, an Electron Acceptor Having Antifungal Activity(Biological Chemistry)
- o-Aminodiphenyl-acetic Acid Test as a Spectrophotometric Method for Determination of Pentose Phosphate Esters(Biological Chemistry)
- Characterization of L-Arabinose-induced Bulge Formation in Escherichia coli IFO 3545 Using L-Arabinose-negative Mutants(Microbiology & Fermentation Industry)
- Raw Starch-utilizing Anaerobe from Mesophilic Methane Sludge and Its Production of Amylase(Microbiology & Fermentation Industry)
- Bulge Formation in Escherichia coli Induced by L-Arabinose under Hypertonic Conditions with Sodium Chloride
- Characterization of Xylan-utilizing Anaerobes from Mesophilic and Thermophilic Methane Sludge and Their Xylan Degrading Enzymes(Microbiology & Fermentation Industry)
- Mesophilic and Thermophilic Methane Fermentation of Agro-Wastes and Grasses
- Selective Cytotoxic Effects of 5-Fluorouridine 5'-Diphosphate Sugars on Virus-Transformed Malignant Cells of Mouse Fibroblast
- Existence of an Optically Heterogeneous Peptide Unit in Poly(γ-Glutamic Acid) Produced by Bacillus subtilis
- A Bacteriolytic Effect of UDP-Sugar Hydrolase on Escherichia coli via Its Overproduction Using a Modified ushA Gene Expression System
- Methane Fermentation of Ramie-Refining Waste Water
- Synergistic fungicidal activities of polymyxin B and ionophores, and their dependence on direct disruptive action of polymyxin B on fungal vacuole
- Antimycin A-induced cell death depends on AIF translocation through NO production and PARP activation and is not involved in ROS generation, cytochrome c release and caspase-3 activation in HL-60 cells
- Enhancement effect of N-methyl-N"-dodecylguanidine on the vacuole-targeting fungicidal activity of amphotericin B against the pathogenic fungus Candida albicans
- The vacuole-targeting fungicidal activity of amphotericin B against the pathogenic fungus Candida albicans and its enhancement by allicin
- Dependence of vacuole disruption and independence of potassium ion efflux in fungicidal activity induced by combination of amphotericin B and allicin against Saccharomyces cerevisiae
- Comparative Analysis of Spinal Hemangioblastomas in Sporadic Disease and Von Hippel-Lindau Syndrome