Methane Fermentation of Ramie-Refining Waste Water

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Methane fermentation of ramie-refining waste water was examined by both conventional single-stage digestion and two-phase fermentation using 5 and 20 l fermentors of semi-continuous cultrue, and the following results were obtained.1. Gas production was 4,664 ml (CH_4,54%-68%) per 1 l of waste water for 10 to 17 day's incubation at 37℃ in conventional single-stage digestion. Chemical oxygen demand (COD) of the supernatant of the mash decreased from 4,824 to 450 ppm during the fermentation. Gas production and COD reduction rate constants were calculated to be 0.0306-0.076 day^<-1> and 0.24-0.91×10^<-4> day^<-1>, respectively.2. Two-phase fermentation was examined with an exchange volume of 400-1,600 ml per 2 days in semi-continuous culture. Volatile fatty acids amounting to 3.04 mg per ml accumulated in the mash of the acidogenic phase, and consumption of these acids was observed in the mash of the methanogenic phase. The amount of acetic acid in the effuent from the methanogenic phase was 0.091 mg per ml. The volume of gas evolved was 560-712 ml (CH_4,41%-49%) per day in the acidogenic phase and 246-1371 ml (CH_4,56%-58%) per day in the methanogenic phse. The gas production and COD reduction rate constants were 0.143-0.260 day^<-1> and 1.01-7.53×10^<-4> day^<-1>, respectively. The counts of viable anaerobic microbes in the mash were 1×10^8 per ml in the acidogenic phase and 0.6×10^8 per ml in the methanogenic phase in the volume exchanged (540 ml) of waste water per 2 days. Methanogenic microbes in the mash of the two-phase fermentation were 48% and 77%, respectively, of the totals in these two mashes.
公益社団法人日本生物工学会の論文
1984-04-25

Methane Fermentation of Ramie-Refining Waste Water

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