The Effect of Hemicellulolytic Enzymes on Mesophilic Methane Fermentation

概要

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Mesophilic methane fermentation was examined using soybean seed coat, a waste from soybean processing for oil manufacture, with or without treatment with hemicellulolytic enzymes of Aspergillus niger, and the following results were obtained : 1. The methane fermentation bacteria acclimated to soybean seed coat medium were shown to consume monosaccharides and evolve methane in the following decreasing order : glucose, fructose, mannose≫xylose, galactose, glucosamine, galacturonic acid>arabinose. The bacteria were also shown to form methane from a gas mixture of hydrogen and carbon dioxidc.2. In fermentation of soybean seed coat treated with the fungal enzyme, about 70% of the total sugar content was consumed in four weeks, and the gas evolution was about twice that without the fungal enzyme. The gas evolved was composed of 60% methane and 36% carbon dioxide.In general, vigorous evolution of hydrogen and carbon dioside occurred at a very early stage of fermentation, and was followed by formation of methane. The maximum gas evolution of the enzyme-treated mash took place in 6 days while that of untreated mash occurred one week later. Chemical oxygen demand of the supernatant of the former mash was decreased by fermentation to 7.0% of the initial level.
社団法人日本生物工学会の論文
1977-04-25