Induced Production of Acidic Polysaccharide by Benzalkonium Chloride-resistant Bacterium (Enterobacter species) in the Presence of Hexachlorophen and Chlorhexidine(Microbiology & Fermentation Industry)

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The bacterium, which was isolated from soil and identified as Enterobacter sp., was induced by hexachlorophen (HCP) and chlorhexidine (CH), as well as benzalkonium chloride (BC), to produce acidic polysaccharide. HCP is a bisphenol and CH is a bisbiguanido, while BC is a quarternary ammonium compound. The cells produced the maximum amount of the polysaccharide (0.3〜0.9 mg as total sugar/mg dry weight cells) in a 0.07 M potassium phosphate buffer (pH 7.2) containing 0.22 M glucose and approximately 0.1mM BC or HCP, or 0.06mM CH. There was no growth of the cells in these conditions. The polysaccharides produced in the presence of each drug were all composed of fucose, glucose, galactose and glucuronic acid. At the optimum concentration for polysaccharide production, a large amount of UV-absorbing material was released from the cells.
社団法人日本農芸化学会の論文
1986-10-23