Analysis of Dissociated Single Neurons by Simple and Semi-Quantitative: RT-PCR (Reverse Transcription and Polymerase Chain Reaction)
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概要
- 論文の詳細を見る
We have developed a simple and semi-quantitative method for mRNA determination in single cells using the reverse transcription and polymerase chain reaction (RT-PCR). The distinct features of this method are the highly efficient RNA harvest from whole dissociated cells and the ability to perform all RT procedures in one tube that allowed semi-quantitative determination of mRNA in dissociated cells. This method revealed that histamine H<SUB>1</SUB>-receptor mRNA was highly expressed in 5/28 small and 1/26 large dorsal root ganglion neurons of the mouse.
- 社団法人 日本薬理学会の論文
- 1997-05-01
著者
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ANDOH Tsugunobu
Department of Applied Pharmacology, Graduate School of Medicine and Pharmaceutical Sciences, Univers
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KURAISHI Yasushi
Department of Applied Pharmacology, Graduate School of Medicine and Pharmaceutical Sciences, Univers
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Andoh Tsugunobu
Department Of Applied Pharmacology Faculty Of Pharmaceu Tical Sciences
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Tohda Chihiro
Analytical Research Center For Ethnomedicines Research Institute For Wakan-yaku Toyama Medical And P
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Kuraishi Yasushi
Department Of Applied Pharmacology Faculty Of Pharmaceu Tical Sciences
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Kuraishi Yasushi
Department Of Applide Biochemistry Research Institute For Wakan-yaku Toyama Toyama Medical And Pharm
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Tohda Chihiro
Analytical Research Center For Ethnomedicines Research Institute For Wakan-yaku Toyama Medical And P
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