Development of a New Cultivar-Discrimination Method Based on DNA Polymorphism in a Vegetatively Propagated Crop
スポンサーリンク
概要
- 論文の詳細を見る
Restriction landmark genome scanning (RLGS) is a high-speed genome analysis method to detect DNA polymorphism, and is suitable to develop useful DNA markers for cultivar-discrimination of agricultural plants. The mat-rush, <I>Juncus effusus </I>is a vegetatively propagated crop that has been cultivated for over 500 years in Japan as the material of "tatami-omote," a surface of Japanese conventional mats. In recent years, Japanese mat-rush cultivars are grown in foreign countries and reimported to Japan in violation of the Seeds and Seedlings Law, economically damaging domestic production. The largest problem of illegal mat-rush reimportation has been with cv. Hinomidori. We developed a cultivar-discrimination DNA marker of mat-rush using RLGS, and detected the RLGS spot markers that didn′t exist specifically in Hinomidori. The spot marker can be used with the RLGS method to identify Hinomidori accurately among cultivars.
- 独立行政法人 国際農林水産業研究センターの論文
著者
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IIMURE Kazuhiko
Kumamoto Prefectural Agriculture Research Center
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Takamiya Tomoko
Department Of Epidemiology Graduate School Of Public Health. University Of Pittsburgh
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Kawase Makoto
Genebank National Insitute Of Agrobiological Sciences
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Okamoto Hiroyuki
Department Of Agricultural Chemistry Kyoto University
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Saito Akira
Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University
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TOMIOKA Keisuke
Genebank, National Institute of Agrobiological Sciences
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DOMON Eiji
Department of Crop and Food Science, National Agricultural Research Center for Kyushu Okinawa Region
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OHTAKE Yuhko
Department of Plant Biotechnology, National Institute of Agrobiological Sciences
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MURAKAMI Yasufumi
Department of Biological Science & Technology, Faculty of Industrial Science & Technology, Tokyo University of Science
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OKUIZUMI Hisato
Department of Molecular Genetics, National Institute of Agrobiological Sciences
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OKAMOTO Hiroyuki
Department of Molecular Genetics, National Institute of Agrobiological Sciences
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MURAKAMI Yasufumi
Department of Biological Science & Technology, Faculty of Industrial Science & Technology, Tokyo University of Science
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TAKAMIYA Tomoko
Department of Biological Science & Technology, Faculty of Industrial Science & Technology, Tokyo University of Science
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SAITO Akira
Department of Crop and Food Science, National Agricultural Research Center for Kyushu Okinawa Region
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