Application of RLGS Method for Detection of Alteration in Tissue Cultured Plants
スポンサーリンク
概要
- 論文の詳細を見る
Tissue culture is used as a tool to propagate seedlings massively in industry. However, unfavorable genomic alteration that affects phenotypic characters occurs in the procedure. A technique which scans these genetic changes genome-wide can enables us to discriminate the clone that has genomic alteration at early stages. In this study, we used restriction landmark genome scanning (RLGS) to detect alterations among ramets of rice, which redifferentiated from a callus that had been cultured for 4 weeks. As a result, 6% (10 spots) of the total (161 spots examined) were different between two ramets. One spot out of ten was cloned and was located on a predicted gene. Application of a PCR-based method to the RLGS spot region detected one methylation alteration among the ramets. Accordingly, RLGS was successful in surveying methylation alteration events during tissue culture in plants and it should be possible to use this method for monitoring of alteration levels.
- 独立行政法人 国際農林水産業研究センターの論文
著者
-
Kawase Makoto
Genebank National Insitute Of Agrobiological Sciences
-
Ohtake Yuhko
Division Of Biological Sciences Graduate School Of Science Hokkaido University
-
OKUIZUMI Hisato
Genebank, National Institute of Agrobiological Sciences
-
MURAKAMI Yasufumi
Faculty of Industrial Science & Technology, Tokyo University of Science
-
TAKAMIYA Tomoko
Faculty of Industrial Science & Technology, Tokyo University of Science
-
HOSOBUCHI Saeko
Genebank, National Institute of Agrobiological Sciences
-
NOGUCHI Tomotsugu
Faculty of Industrial Science & Technology, Tokyo University of Science
-
NOGUCHI Tomotsugu
Faculty of Industrial Science & Technology, Tokyo University of Science
-
OHTAKE Yuhko
Division of Plant Sciences, National Institute of Agrobiological Sciences
関連論文
- Development of mini core collection of Japanese rice landrace
- Development of an RFLP-based Rice Diversity Research Set of Germplasm
- Molecular cloning and characterization of granule bound starch synthase I cDNA from a grain amaranth (Amaranthus cruentus L.)
- Salicylic Acid Induces the Expression of a Number of Receptor-Like Kinase Genes in Arabidopsis thaliana
- Development of a New Cultivar-Discrimination Method Based on DNA Polymorphism in a Vegetatively Propagated Crop
- Application of RLGS Method for Detection of Alteration in Tissue Cultured Plants
- Genetic Relationships of Sorghum Germplasm in Asia and Africa Revealed by Rice cDNA-STS and Indel Markers