Efficient Establishment of Pig Embryonic Fibroblast Cell Lines with Conditional Expression of the Simian Vacuolating Virus 40 Large T Fragment
スポンサーリンク
概要
- 論文の詳細を見る
The pig is an important animal for both agricultural and medical purposes. However, the number of pig-derived cell lines is relatively limited when compared with mouse- and human-derived lines. We established in this study a retroviral conditional expression system for the Simian vacuolating virus 40 large T fragment (SV40T) which allowed us to efficiently establish pig embryonic fibroblast cell lines. The established cell lines showed high levels of cell proliferation and resistance to cellular senescence. A chromosome analysis showed that 84% of the cells had the normal karyotype. Transient expression of the Cre recombinase allowed us to excise the SV40T fragment from the genome. The development of this research tool will enable us to quickly establish new cell lines derived from various animals.
著者
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Sato Eimei
Laboratory Of Animal Reproduction Graduate School Of Agricultural Science Tohoku University
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Miyazawa Teruo
Food & Biodynamic Chemistry Laboratory Graduate School Of Life Science And Agriculture Tohoku Un
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Nakagawa Kiyotaka
Food & Biodynamic Chemistry Laboratory Graduate School Of Life Science And Agriculture Tohoku Un
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Hoshino Yumi
Laboratory Of Animal Reproduction Graduate School Of Agricultural Science Tohoku University
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Uchida Takafumi
Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc
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Nishimori Katsuhiko
Laboratory Of Molecular Biology Department Of Molecular And Cell Biology Graduate School Of Agricult
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Ito Hisao
Departmen Of Radiology School Of Medicine Keio University
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HANADA Hirofumi
Department of Animal Breeding and Genetics, National Institute of Animal Industry
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Katayama Masafumi
Laboratory Of Animal Breeding And Genetics Graduate School Of Agricultural Science Tohoku University
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Fukuda Tomokazu
Laboratory Of Animal Breeding And Genetics Graduate School Of Agricultural Science Tohoku University
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Katayama Masafumi
Laboratory Of Animal Genetics Graduate School Of Agricultural Sciences Tohoku University
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Komagata Homika
Department Of Animal Science Tokyo University Of Agriculture
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Eitsuka Takahiro
Department Of Food Science Niigata University Of Pharmacy And Applied Life Sciences
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Mizukami Hiroaki
Center For Molecular Medicine Jichi Medical University
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Song Sanghoun
Laboratory Of Animal Physiology Graduate School Of Agricultural Sciences Tohoku University
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Roh Sanggun
Laboratory Of Animal Physiology Graduate School Of Agricultural Sciences Tohoku University
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Yoshizawa Takayuki
Laboratory Of Animal Genetics Graduate School Of Agricultural Sciences Tohoku University
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