凍結融解・洗浄精子の頚管粘液添加培地内移動能による雄ウシの受胎性評価
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概要
- 論文の詳細を見る
To investigate a means for evaluating bull fertility, analysis of frozen-thawed bull sperm migration in the medium with or without bovine cervical mucus was carried out. Sperm were prepared by thawing frozen-semen from 4 bulls, which showed different conception rates of 71, 43, 43 and 1% by non-return rate post AI. Media with 50% cervical mucus and with 1% BSA were prepared to make a test column. A horizontal column of 21 cm length had specific shape and composition on a 60-mm Petri dish, and was used to analyze the migration characteristics of the sperm. After 2 h incubation, viability and migration of the sperm were examined. There was correlation between bull fertility and sperm migration in the media containing cervical mucus: the migration in the medium with cervical mucus of sperm from the bull with low fertility was lower than those of the other bulls with high fertility. After a single wash, the migration in medium without cervical mucus of sperm with high fertility was significantly higher than that of sperm with low fertility. The migration of sperm increased with the number of washes. This study confirmed that sperm viability after migration in a medium varied with different media used and was positively correlation with bull fertility in the medium containing cervical mucus. We concluded that the Petri dish-based horizontal column may be a useful tool for analyzing sperm migration as a predictor of bull fertility.
- 日本繁殖生物学会の論文
著者
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SASADA Hiroshi
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
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SATO Eimei
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
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Sato Eimei
Laboratory Of Animal Reproduction Graduate School Of Agricultural Science Tohoku University
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濱野 光市
信州大学 農学部
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濱野 光市
信州大学農学部附属農場
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濱野 光市
信州大学附属農場
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濱野 光市
日本農産工業
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Tsujii Hirotada
Faculty of Agriculture, University of Shinshu, Kamiina-gun, Nagano 399-45, Japan
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濱野 光市
Faculty of Agriculture, Shinshu University
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Sasada Hiroshi
Laboratory Of Animal Reproduction Department Of Animal Science Faculty Of Agriculture Tohoku Univers
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田中 沙知
Faculty of Agriculture, Shinshu University
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川名 陽子
Faculty of Agriculture, Shinshu University
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TAKAHASHI Tatsuo
Nagano Prefectural Animal Industry Experiment Station
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MIYAWAKI Koh-hei
Nagano Prefectural Animal Industry Experiment Station
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ARIMA Hiroshi
Faculty of Agriculture, Shinshu University
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濱野 光市
静岡大学 農学部
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Hamano Koh-ichi
Faculty Of Agriculture Shinshu University
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Tsujii Hirotada
Faculty Of Agriculture Shinshu University
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TANAKA Sachi
Faculty of Agriculture, Shinshu University
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KAWANA Yoko
Faculty of Agriculture, Shinshu University
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Arima Hiroshi
Faculty Of Agriculture Shinshu University
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