Acetylation Level of Histone H3 in Early Embryonic Stages Affects Subsequent Development of Miniature Pig Somatic Cell Nuclear Transfer Embryos
スポンサーリンク
概要
- 論文の詳細を見る
Successful cloning by somatic cell nuclear transfer (SCNT) requires a reprogramming process in which the epigenetic state of a differentiated donor nucleus must be converted into an embryonic totipotent state. However, this epigenetic reprogramming is incomplete in SCNT embryos, causing low production efficiency. Recently, it has been reported that trichostatin A (TSA), an inhibitor of histone deacetylase, potentially enhances cloning efficiency. The aim of the present study was to optimize the TSA treatment for miniature pig SCNT embryos and investigate the effect of the acetylation level of histone on developmental competence of SCNT embryos. In order to optimize the TSA treatment, we examined the developmental competence of SCNT embryos under various exposure times (0-50 h) and concentrations (0-500 nM). Treatment with 5 nM TSA for 15 and 20 h beginning at the start of activation significantly increased the blastocyst formation rate (34.6 and 32.4 vs. 18.2%, respectively) and mean cell number (57.0 ± 2.7 and 56.6 ± 2.7 vs. 43.5 ± 2.1, respectively) as compared with the non-treated group (0 h). We then investigated the acetylation levels of histone H3 in SCNT embryos treated with or without TSA (TSA (+) or TSA (-)) as compared with in vitro- fertilized (IVF) embryos. The acetylation levels of the TSA (-) SCNT embryos at the pseudo-pronuclear and 2-cell stages were significantly lower than those of the IVF embryos at the same developmental stages. In contrast, the acetylation levels of the TSA (+) SCNT embryos were similar to those of the IVF embryos. There was no difference in the acetylation levels of all groups at the blastocyst stage. Our data therefore suggests that the acetylation level of histone H3 at the pseudo-pronuclear and 2-cell stages is positively correlated with subsequent development of SCNT embryos, which may be an important event for the vital development of SCNT embryos in miniature pigs.
- 2009-12-01
著者
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SATO Eimei
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
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KAWAHARA Manabu
Laboratory of Animal Resource Development, Faculty of Agriculture, Saga University
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Sato Eimei
Tohoku Univ. Sendai Jpn
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Sato E
Graduate School Of Agricultural Science Tohoku University
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Sato Eimei
東京大学医科学研究所
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Sato Eimei
Laboratory Of Animal Reproduction Graduate School Of Agricultural Science Tohoku University
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YAMANAKA Ken-ichi
National Agricultural Research Center for Kyushu Okinawa Region (KONARC)
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Sato Eimei
Graduate School Of Agricultural Science Tohoku University
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SUGIMURA Satoshi
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
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WAKAI Takuya
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
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Sato E
Univ. Tokyo Tokyo Jpn
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Kawahara Manabu
Laboratory Of Animal Reproduction Graduate School Of Agricultural Science Tohoku University
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Yamanaka Ken-ichi
National Agricultural Res. Center For Kyushu Okinawa Region
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Yamanaka Ken-ichi
National Agricultural Research Center For Kyushu Okinawa Region
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Kawahara Manabu
佐賀大学 農学部動物資源開発学分野
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Wakai Takuya
Laboratory Of Animal Reproduction Graduate School Of Agricultural Science Tohoku University
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WAKAI Takuya
Department of Veterinary and Animal Sciences, University of Massachusetts
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Wakai Takuya
Department Of Veterinary And Animal Sciences University Of Massachusetts
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Sugimura Satoshi
Laboratory Of Animal Reproduction Graduate School Of Agricultural Science Tohoku University
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Kawahara Manabu
Laboratory Of Animal Breeding And Reproduction Graduate School Of Agriculture Hokkaido University
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WAKAI Takuya
Department of Bioscience, Tokyo University of Agriculture
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