Roles of Genes Regulated by Two Paternally Methylated Imprinted Regions on Chromosomes 7 and 12 in Mouse Ontogeny
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概要
- 論文の詳細を見る
We studied the longevity of mice produced without sperm using the genomes of oocytes that are already committed to a germline cell lineage. The first sperm-free mouse “KAGUYA”, which we term ‘bi-maternal mouse’, was born on 3 February, 2003. Bi-maternal embryos were generated using 2 sets of female genomes—one derived from fully grown oocytes from normal adults and the other from non-growing oocytes from newborn pups. These genomes were combined by nuclear transfer. We refined the technique for generating bi-maternal mice and found that genetic manipulations in only 2 regions—the imprinting centres of Igf2-H19 and Dlk1-Gtl2—on chromosomes 7 and 12 of the newborn pups allowed us to generate bi-maternal mice at a high rate. Studying bi-maternal conceptuses and mice provides further insight into the mechanisms by which paternally methylated imprinted genes regulate mammalian ontogenesis.
- 2012-04-01
著者
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KONO Tomohiro
Department of BioScience, Tokyo University of Agriculture
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Kono Tomohiro
Department Of Animal Science Tokyo University Of Agriculture
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Kono Tomohiro
Dep. Of Bioscience Tokyo Univ. Of Agriculture Tokyo 156-8502 Jpn
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Kawahara Manabu
Laboratory Of Animal Breeding And Reproduction Graduate School Of Agriculture Hokkaido University
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- Identification of a New Imprinted Gene, Rian, on Mouse Chromosome 12 by Fluorescent Differential Display Screening
- Production of Functional Oocytes In Vitro
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- Preface
- DNA Synthesis in Mouse 1-Cell Embryos Containing Transferred Nuclei
- Epigenetically Immature Oocytes Lead to Loss of Imprinting During Embryogenesis
- Production of Live Young by Serial Nuclear Transfer with Mitotic Stages of Donor Nuclei in Mice
- Roles of Genes Regulated by Two Paternally Methylated Imprinted Regions on Chromosomes 7 and 12 in Mouse Ontogeny
- Determination by Real-Time RT-PCR of Imprinted Expression of the Insulin-Like Growth Factor II (Igf2) Gene in Mouse Uniparental Fetuses.
- Comparison of the RNA Polymerase I-, II- and III-Dependent Transcript Levels Between Nuclear Transfer and In Vitro Fertilized Embryos at the Blastocyst Stage
- RNF12 is a Primary Factor for Xist Activation in Mouse Somatic Cloned Embryos