Epigenetically Immature Oocytes Lead to Loss of Imprinting During Embryogenesis

スポンサーリンク

概要

• 論文の詳細を見る

• Loss of imprinting (LOI) is occasionally observed in human imprinting disorders. However, the process behind the LOI is not fully understood. To gain a better understanding, we produced embryos and pups from mouse oocytes that lacked a complete methylation imprint using a method that involved transferring the nuclei of growing oocytes into the cytoplasm of enucleated fully grown oocytes following in vitro fertilization (IVF). We then analyzed the imprinting statuses. Our findings show that the incomplete methylation imprint derived from growing oocytes results in epigenetic mosaicism or a loss of methylation imprint (LOM) at maternal alleles in embryos. In some embryos, both hypo- and hypermethylated maternal Kcnq1ot1 alleles were detected, whereas either hypo- or hypermethylated maternal Kcnq1ot1 alleles were detected in others. Such tendencies were also observed at the Igf2r and Mest loci. Gene expression levels of imprinted genes were linked with their methylation statuses in some but not all embryos. Possible explanations of the inconsistency between the data from DNA methylation and gene expression include epigenetic mosaicism in embryos. Pups were successfully produced from growing oocytes at a quite low frequency. They exhibited an obese phenotype and LOI with respect to Igf2r, Snrpn and Mest. Our finding suggests the possibility that LOI/LOM at maternal alleles in human concepti could be derived from epigenetically immature/mutated oocytes.

• 2011-06-01

著者

• Obata Yayoi

  Department Of Bioscience Tokyo University Of Agriculture

• Hiura Hitoshi

  Department Of Information And Computer Sciences Toyohashi University Of Technology:(present Office)n

• Kono Tomohiro

  Department Of Bioscience Tokyo University Of Agriculture

• Kono Tomohiro

  Department Of Animal Science Tokyo University Of Agriculture

• FUKUDA Atsushi

  Department of Physical Electronics, Tokyo Institute of Technology

• KOMIYAMA Junichi

  Department of BioScience, Tokyo University of Agriculture

• HATADA Izuho

  Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University

• Obata Yayoi

  Dep. Of Bioscience Tokyo Univ. Of Agriculture

• Hatada Izuho

  Biological Genome Resource Center Institute For Molecular And Cellular Regulation Gunma University

• Hatada Izuho

  Biosignal Genome Resource Center Institute For Molecular And Cellular Regulation Gunma University

• Fukuda Atsushi

  Department Of Bioscience Tokyo University Of Agriculture

• Komiyama Junichi

  Department Of Bioscience Tokyo University Of Agriculture

• Hiura Hitoshi

  Department Of Bioscience Tokyo University Of Agriculture

• Kono Tomohiro

  Dep. Of Bioscience Tokyo Univ. Of Agriculture Tokyo 156-8502 Jpn

関連論文

• Involvement of Insulin-like Growth Factor 2 in Angiogenic Factor Transcription in Bi-maternal Mouse Conceptuses
• Enhanced Washout of I-123 Beta-Methyl-lodophenyl Pentadecanoic Acid (BMIPP) Predicts Good Left Ventricular Functional Recovery after Acute Anterior Myocardial Infarction
• Epigenetically Immature Oocytes Lead to Loss of Imprinting During Embryogenesis
• Direct Production of Gene-targeted Mice from ES Cells by Nuclear Transfer and Gene Transmission to their Progeny
• Prediction of long-term cardiac events by I123-MIBG imaging after acute myocardial infarction and reperfusion therapy
• Wavelength Dependence of Optical Waveguide-Type Devices for Recognition of QPSK Routing Labels
• Synergistic Role of Igf2 and Dlk1 in Fetal Liver Development and Hematopoiesis in Bi-Maternal Mice
• Synergistic Role of Igf2 and Dlk1 in Fetal Liver Development and Hematopoiesis in Bi-Maternal Mice
• Birth of Parthenogenetic Mouse(Symposium of the Process of Fertilization-From Sex Determination to Epigenetics,Symposium,PROCEEDING OF THE 76^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)
• Optical Label Recognition Using Tree-Structure Self-Routing Circuits Consisting of Asymmetric X-Junctions(Optoelectronics)
• All-Optical Label Recognition Using Self-Routing Architecture of Mach-Zehnder Interferometer Optical Switches with Semiconductor Optical Amplifiers(Optoelectronics)
• RT-PCRによるウシ胎子におけるIgf2ならびにIgf2r遺伝子の発現解析
• 胎齢期にあるクローンマウスで観察された肥大した胎盤および開眼の組織学的研究
• Surface Potential of Heat-Treated PI Langmuir-Blodgett Films Deposited on Metal Electrodes
• Identification of a New Imprinted Gene, Rian, on Mouse Chromosome 12 by Fluorescent Differential Display Screening
• A microarray-based method for detecting methylated loci
• Production of Functional Oocytes In Vitro
• Charge Storage Phenomena and I-V Characteristics Observed in Ultrathin Polyimide Langmuir-Blodgett Films
• Long-Term Effects of In Vitro Growth of Mouse Oocytes on Their Maturation and Development
• Preface
• Photoinduced Displacement Current in Polyimide Langmuir-Biodgett Films Deposited on Au Electrodes
• Photoinduced Current across Polyimide Langmuir-Blodgett Films in the Absence of an External Electric Field
• Study on the Mechanism of Maternal Imprinting During Oocyte Growth
• Epigenetically Immature Oocytes Lead to Loss of Imprinting During Embryogenesis
• Chondroblastic Osteosarcoma Arising from the Pleura : Report of a Case
• INVESTIGATING TRAVEL BEHAVIOR ASSOCIATED WITH THE INTRODUCTION OF CAR-SHARING SYSTEM IN BANGKOK
• Photoinduced Surface Poterutial in Polyimide Langmuir-Blodgett Films
• Roles of Genes Regulated by Two Paternally Methylated Imprinted Regions on Chromosomes 7 and 12 in Mouse Ontogeny
• ANALYSIS OF CAR SHARING APPLICATION ON CONSUMER ORIENTATION AND THEIR MODAL SELECTION IN BANGKOK
• Determination by Real-Time RT-PCR of Imprinted Expression of the Insulin-Like Growth Factor II (Igf2) Gene in Mouse Uniparental Fetuses.
• Comparison of the RNA Polymerase I-, II- and III-Dependent Transcript Levels Between Nuclear Transfer and In Vitro Fertilized Embryos at the Blastocyst Stage
• RNF12 is a Primary Factor for Xist Activation in Mouse Somatic Cloned Embryos

もっと見る 閉じる

スポンサーリンク