Determination by Real-Time RT-PCR of Imprinted Expression of the Insulin-Like Growth Factor II (Igf2) Gene in Mouse Uniparental Fetuses.
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概要
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Uniparental mouse embryos are generally used in the study of imprinting mechanisms as models to determine parental expression of imprinted genes. In this study, we used the real-time RT-PCR method to carry out a quantitative analysis of the expression of the insulin-like growth factor II (Igf2) gene, which is imprinted and expressed solely from the paternal allele, in androgenetic and parthenogenetic fetuses at day 9.5 of gestation. The mean expression, relative to that of control biparental fetuses, was detected to be 319% (90 to 585%) in androgenetic fetuses and 5.9% (3.7 to 9.5%) in parthenogenetic fetuses. The present results confirm that parental-specific expression of the Igf2 gene is maintained in uniparental fetuses, and also show that the real-time RT-PCR procedure is an effective method for quantitative analysis of gene expression using amounts of mRNA that are too small for other methods to detect.
- 日本繁殖生物学会の論文
著者
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Sotomaru Yusuke
Department Of Bioscience Tokyo University Of Agriculture
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Kono Tomohiro
Department Of Animal Science Tokyo University Of Agriculture
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DOMEKI Ikuo
Department of Animal Reproduction, Faculty of Agriculture, Tokyo University of Agriculture
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KONO Tomohiro
Department of Animal Science, Tokyo University of Agriculture
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KATSUZAWA Yukiko
Department of Animal Science, Tokyo University of Agriculture
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- Preface
- Solid-phase Extraction of Estradiol-17β from Bovine Blood Plasma for its Peripheral Detection by Radioimmunoassay
- Epigenetically Immature Oocytes Lead to Loss of Imprinting During Embryogenesis
- Roles of Genes Regulated by Two Paternally Methylated Imprinted Regions on Chromosomes 7 and 12 in Mouse Ontogeny
- Determination by Real-Time RT-PCR of Imprinted Expression of the Insulin-Like Growth Factor II (Igf2) Gene in Mouse Uniparental Fetuses.
- Comparison of the RNA Polymerase I-, II- and III-Dependent Transcript Levels Between Nuclear Transfer and In Vitro Fertilized Embryos at the Blastocyst Stage
- RNF12 is a Primary Factor for Xist Activation in Mouse Somatic Cloned Embryos