Long-Term Effects of In Vitro Growth of Mouse Oocytes on Their Maturation and Development
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概要
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Since very few oocytes grow completely in vivo, in vitro growth (IVG) of ovarian oocytes may provide a new source of functional oocytes. The long-term effects of in vitro maturation (IVM) of oocytes and in vitro culture of fertilized eggs have been reported; however, the effects of IVG of oocytes are unknown. Here in, we report the long-term effects of IVG of oocytes. Ovaries from 1-day-old mice containing non-growing oocytes were cultured for 10 days; the isolated follicles were then cultured for 11 days. Secondary follicles from 10-day-old mice were also cultured for 11 days. The nuclei of oocytes collected from the IVG and Graafian follicles of adult mice were transferred to enucleated oocytes grown in vivo, respectively. Developmental competence was examined following IVM of the reconstituted oocytes. Chronologically, oocytes of 1-day-old, 10-day-old and adult mice were cultured for 22, 12 and 1 day(s). The result showed that the reconstituted eggs developed into pups at high rates after nuclear transfer and in vitro fertilization (IVF) in all the experimental groups (29%-45%). However, the pups from reconstituted eggs containing the nuclei of 22-day cultured oocytes were heavier than the control pups (P < 0.05). We concluded that long-term culture of oocytes did not affect their nuclear ability to develop to term; however, fetal growth was affected by the culture duration or culture conditions during the initial phase of follicular growth.
- 日本繁殖生物学会の論文
- 2007-12-01
著者
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KONO Tomohiro
Department of BioScience, Tokyo University of Agriculture
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Obata Yayoi
Department of BioScience, Tokyo University of Agriculture BRAIN
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Obata Yayoi
Department Of Bioscience Tokyo University Of Agriculture
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Kono Tomohiro
Department Of Bioscience Tokyo University Of Agriculture
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Kono Tomohiro
Department Of Animal Science Tokyo University Of Agriculture
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HATADA Izuho
Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University
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Obata Yayoi
Dep. Of Bioscience Tokyo Univ. Of Agriculture
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MAEDA Yuhei
Department of Animal Science, Tokyo University of Agriculture
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HATADA Izuho
Biological Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University
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Hatada Izuho
Biological Genome Resource Center Institute For Molecular And Cellular Regulation Gunma University
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Maeda Yuhei
Department Of Animal Science Tokyo University Of Agriculture
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Kono Tomohiro
Dep. Of Bioscience Tokyo Univ. Of Agriculture Tokyo 156-8502 Jpn
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- Preface
- DNA Synthesis in Mouse 1-Cell Embryos Containing Transferred Nuclei
- Study on the Mechanism of Maternal Imprinting During Oocyte Growth
- Epigenetically Immature Oocytes Lead to Loss of Imprinting During Embryogenesis
- Production of Live Young by Serial Nuclear Transfer with Mitotic Stages of Donor Nuclei in Mice
- Roles of Genes Regulated by Two Paternally Methylated Imprinted Regions on Chromosomes 7 and 12 in Mouse Ontogeny
- Determination by Real-Time RT-PCR of Imprinted Expression of the Insulin-Like Growth Factor II (Igf2) Gene in Mouse Uniparental Fetuses.
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- RNF12 is a Primary Factor for Xist Activation in Mouse Somatic Cloned Embryos