未成熟ブタの卵胞発育過程における肝細胞増殖因子(HGF)とアンギオポエチンおよびそれらの受容体のメッセンジャーRNA発現
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概要
- 論文の詳細を見る
Angiogenic factors are associated with angiogenesis during follicular development in the mammalian ovary. The aim of the present study was to determine the relationships between the vascular network and mRNA expressions of angiopoietins (Ang)-1, Ang-2 and hepatocyte growth factor (HGF), and their receptors in follicles at different developmental stages during follicular development. Ovaries in gilts were collected 72 h after equine chorionic gonadotropin (eCG, 1250 IU) treatment for histological observation of the capillary network. Granulosa cells and thecal tissues in small (<4 mm), medium (4-5 mm) or large (>5 mm) individual follicles were collected for detection of mRNA expression of HGF, Ang-1 and Ang-2 in granulosa cells, and HGF receptor (HGF-R) and Tie-2 in the theca cells by semi-quantitative RT-PCR. The number of capillaries in the thecal cell layer increased significantly in healthy follicles at all developmental stages in the eCG group compared with those in controls. The expression of Ang-1 mRNA declined in granulosa cells of medium and large follicles and the level of Ang-2 mRNA increased in granulosa cells of small follicles after eCG treatment. The ratio of Ang-2/Ang-1 increased in small, medium and large follicles from ovaries after eCG treatment, but Tie-2 mRNA expression in the theca cells did not change. The level of HGF mRNA increased in granulosa cells of small follicles after eCG treatment but HGF-R in theca cells was not increased by eCG. These data suggested that the angiopoietins might be associated with thecal angiogenesis during follicular development in eCG-treated gilts.
- 日本繁殖生物学会の論文
著者
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佐々田 比呂志
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
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佐々田 比呂志
宮城農業短期大学
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SATO Eimei
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
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Sato Eimei
Laboratory Of Animal Reproduction Graduate School Of Agricultural Science Tohoku University
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飯島 康仁
東北大学大学院農学研究科
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清水 隆
帯広畜産大学大学院畜産衛生学専攻生殖科学研究室
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清水 隆
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
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飯島 康仁
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
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Sasada Hiroshi
Laboratory Of Animal Reproduction Department Of Animal Science Faculty Of Agriculture Tohoku Univers
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SHIMIZU Takashi
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
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IIJIMA Koji
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
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