Effect of Relaxin on In Vitro Fertilization of Porcine Oocytes
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概要
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Relaxin is a peptide hormone belonging to the insulin super family that has a variety of biological functions. The present experiment was designed to investigate the effects of relaxin on sperm function and on in vitro fertilization (IVF) of porcine oocytes. Porcine spermatozoa were washed, swum-up, and incubated for 1-4 h in mTALP medium supplemented with 0, 20, or 50 ng/ml relaxin. Motility was determined by observing the type of forward movement of the spermatozoa, and acrosome status was evaluated by applying the triple staining technique. Immature oocytes were aspirated from antral follicles and matured in IVM medium (modified NCSU-37). Matured oocytes were co-cultured with spermatozoa in IVF medium (mTALP) supplemented with 0, 5, 10, 15, or 20 ng/ml relaxin. After 6 h of sperm-oocyte co-incubation, putative zygotes were cultured for 18 h in oocyte culture medium NCSU-37 and then assessed for the rates of monospermy, polyspermy, and male pronucleus formation after acetic orcein staining. Relaxin improved (P<0.05) sperm motility and increased the percentage of acrosome-reacted live spermatozoa during 1-4 h of incubation, although viability was not significantly improved. Significantly (P<0.05) the highest percentage of monospermic (31.7%) and lowest percentage of polyspermic (16.5%) fertilization was achieved from the sperm-oocyte co-culture group treated with 20 ng/ml relaxin as compared to other groups. The percentage of male pronucleus formation was significantly (P<0.05) greater in the 20 ng/ml relaxin sperm-oocyte co-culture group than in the other groups. These results indicate that supplementation with relaxin is capable of improving sperm function and fertilization of porcine oocytes in vitro.
- 2006-10-01
著者
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YOSHIDA Mayumi
Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology, Shi
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TSUJII Hirotada
Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology, Shi
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MIAH A.
Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology, Shi
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HAMANO Ko-ichi
Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology, Shi
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KOHSAKA Tetsuya
Laboratory of Animal Reproduction, Faculty of Agriculture, Shizuoka University
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HAN Young-Joon
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
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SASADA Hiroshi
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
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Tsujii Hirotada
信州大学 総合工学系研究科動物生殖学
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Kohsaka Tetsuya
Laboratory Of Animal Reproduction Faculty Of Agriculture Shizuoka University
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Miah A.
Laboratory Of Animal Biotechnology Interdisciplinary Graduate School Of Science And Technology Shins
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Tsujii Hirotada
信州大学 農学部生物資源開発学講座
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Tsujii Hirotada
Laboratory Of Animal Biotechnology Faculty Of Agriculture Shinshu University
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Hamano Ko-ichi
Laboratory Of Animal Biotechnology Interdisciplinary Graduate School Of Science And Technology Shins
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Han Young-joon
Laboratory Of Animal Reproduction Graduate School Of Agricultural Science Tohoku University
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Sasada Hiroshi
Laboratory Of Animal Reproduction Department Of Animal Science Faculty Of Agriculture Tohoku Univers
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Yoshida Mayumi
Laboratory Of Animal Biotechnology Interdisciplinary Graduate School Of Science And Technology Shins
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Yoshida Mayumi
Laboratory Of Animal Biotechnology Interdisciplinary Graduate School Of Science And Technology Shins
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