Effect of Relaxin on Acrosome Reaction and Utilization of Glucose in Boar Spermatozoa
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概要
- 論文の詳細を見る
Relaxin is a peptide hormone found in seminal plasma that has a physiological influence on sperm motility in some species. There are no reports on the effect of relaxin on acrosome reaction and utilization of glucose in boar spermatozoa. In this study, to investigate the effects of relaxin on sperm motility, acrosome reaction, and incorporation and oxidation of labeled glucose, boar spermatozoa were washed and preincubated for swim-up and then incubated (0-6 h) with 0, 20, or 40 ng/ml relaxin in mTALP medium. The results indicated that the addition of relaxin stimulated sperm motility significantly (P<0.05) during 1-4 h of incubation. The percentage of acrosome-reacted live spermatozoa was higher (P<0.05) when the spermatozoa were treated with 20 or 40 ng/ml relaxin. The rate of incorporation, and oxidation of glucose were also greater (P<0.05) in the spermatozoa incubated with relaxin compared to the control spermatozoa. The rate of incorporation and oxidation of 14C-glucose were increased in correlation with acrosome reaction up to 4 h of incubation and then decreased in line with the increasing incubation period. In conclusion, the present study demonstrates that relaxin accelerates not only motility but also the acrosome reaction and utilization of glucose in boar spermatozoa.
- 2006-12-01
著者
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TSUJII Hirotada
Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology, Shi
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MIAH A.
Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology, Shi
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TAREQ K.
Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology, Shi
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HAMANO Ko-ichi
Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology, Shi
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KOHSAKA Tetsuya
Laboratory of Animal Reproduction, Faculty of Agriculture, Shizuoka University
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Tsujii Hirotada
信州大学 総合工学系研究科動物生殖学
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Tareq K.
Laboratory Of Animal Biotechnology Interdisciplinary Graduate School Of Science And Technology Shins
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Kohsaka Tetsuya
Laboratory Of Animal Reproduction Faculty Of Agriculture Shizuoka University
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Miah A.
Laboratory Of Animal Biotechnology Interdisciplinary Graduate School Of Science And Technology Shins
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Tsujii Hirotada
信州大学 農学部生物資源開発学講座
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Tsujii Hirotada
Laboratory Of Animal Biotechnology Faculty Of Agriculture Shinshu University
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Hamano Ko-ichi
Laboratory Of Animal Biotechnology Interdisciplinary Graduate School Of Science And Technology Shins
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Tareq K.
Laboratory Of Animal Biotechnology Faculty Of Agriculture Interdisciplinary Graduate School Of Scien
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