洗浄による貝類および魚類の筋原線維とミオシンBからのアクチンの損失

概要

論文の詳細を見る
When myofibrils were prepared from various muscles of shell-fishes and of fishes by repeated washing with low ionic strength medium, more actin was released from the shell-fish myofibrils rather than from the fish myofibrils. This conclusion was based on the following results. (1) The inactivation mode of Ca-ATPase activity changed from the typical fist-order process to biphasic first-ored behavior: the rate for inactvation increased as the washing repeated. (2) The typical fisrt-ored inactivation of Ca-ATPase was reversibly restored by the addition of rabbit skeletal F-actin to the washed myofibrils. (3) A component coresponding to acin on the SDS-gel electro-phoresis was specifically decreased in the washed myofibrils, while it was increased in the supernatant and precipitated upon complexing the myosin. The same results show that the loss of actin occurred more readily in the fish dorsal musule myosin B than in the rabbit skeletal muscle myosin B.
日本水産學會の論文