ホタテガイ閉殻筋横紋部からアクチンの調製とその生化学的性質について

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An attempt was successively made to prepare G-actin from scallop striated adductor muscle by using the original method of STRAUB. 1)Crude G-actin was extracted from the acetone dried muscle powder of scallop striated adductor muscle in the same yield as from fish dorsal and rabbit skeletal muscles. This G-actin preparation was found to be contaminated with a subunit corresponding to tropomyosin and one or two other unknown proteins on SDS-gel electrophoresis. 2) It was found that ultracentrifugation of crude F-actin in 0.6M KCl-1 mM MgCl2 at 100, 000g for 120 minutes effectively eliminates the contaminants, while the ordinary reversible polymerization performed in the presence of 0.1M KCl-1 mM MgCl2 and gel filtration were ineffective.3) The purified G-actin retains virtually the same ability to polymerize into F-actin as fish dorsal and rabbit skeletal G-actins upon the addition of 0.1M KCl or 0.1M KCl plus 1 mM MgCl2. 4) F-actin, obtained in such a way was shown to complex with rabbit skeletal myosin by the fact that reconstituted actomyosin had the identical characteristics of natural actomyosin, that is, positive superprecipitation, high ATP-sensitivity, activation of Mg2+-ATPase activity, and stabilization of Ca2+-ATPsae activity, against heat. 5) From these results, it was concluded that the biological activities of scallop striated adductor actin are essentially the same as those of fish dorsal and rabbit skeletal actins.
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ホタテガイ閉殻筋横紋部からアクチンの調製とその生化学的性質について

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