ウバガイ,ホタテガイおよびアカザラガイ閉殻筋アクトミオシンの性質の比較について

概要

論文の詳細を見る
Attempts have been made to prepare actomyosins from the adductor muscle of some sea-shells (akazara scallop, giant ezo scallop, and surf-clam). 1) Actomyosins prepared in this experiment showed all the typical properties of actomyosin as already known on rabbit skeletal muscle; that is, high ATP hydrolyzing activity, superprecipitation (the rate of superprecipitation is slower than that of the rabbit actomyosin), anomalous viscosity and reversible viscosity drip with Mg-ATP. 2) SDS-polyacrylamide gel electrophoretic patterns revealed a little contamination with paramyosin in the actomysoins from the akazara scallop and the giant ezo scallop, and a considerable amount in the surf-clam actomyosin. 3) A maximum activity of Ca+-ATPase was found at KCl concentrations of 0.21M, 0.26M and 0.31M for actomyosins from the giant ezo scallop, the surf-clam and the akazara scallop, respectively. A remarkable increase in Ca2+-ATPase of these actomyosins occurred upon the addition of urea at a final concentration of 1.25M. 4) The first order rate constants for the denaturation of actomyosin Ca2+-ATPase were compared at 30°, 35° and 40°C. The relative stability of the actomyosibns was found to decrease in the order: akazara scallop, surf-clam and giant ezo scallop.
日本水産學會の論文