海獸胃中の蛋白消化酵素に就て(第3報)-I凍結法による酵素溶液の濃縮-(2)鯨PePsinの消化至適温度-

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I) Concentration of the enzyme solution by freezing. We divided the enzyme solution into 5 layers in the test tube, and repeated freezing (in-6° ?? -12°C) and melting (in the room temp.) of it. The concentration of the enzyme in the 5th layer was maximum when it was repeated 5 tiems, and the ratio was ca 3.6. II) The Optimum temperature of the whale-pepsin. The opt. temperature of the whale-pepsin (Preparation from northern rorqual) was found to be 42°C (in 30min., Edestin) and 30°-40°C (in 6min.) This lies in the intermediate between the opt. temp. of the fish which lives in cold water and that of the fish in warmer water.
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