ウバガイおよびホタテガイ平滑閉殻筋から調製したアクチンの性質

概要

論文の詳細を見る
According to the method adopted for striated adductor muscles of scallop, purified actins were successfully prepared from the smooth adductor muscles of surf-clam, Spisula sachalinensis, and scallop, Patinopecten yessoensis. 1) Crude G-actins, extracted by the method of STRAUB, were found to be contaminated with a small amount of tropomyosin and paramyosin. These crude actins were highly purified by the method of SPUDICH et al. The yields of pure G-actins were 400mg and 150mg from 100g of smooth adductor muscles of surf-clam and scallop, respectively. 2) G-actin preparations were polymerized by the addition of 0.1M KCl; they had a viscosity similar to that of rabbit skeletal F-actin. 3) Reconstituted actomyosins from smooth adductor F-actin and rabbit skeletal myosin showed the characteristic properties of actomyosin, i.e. superprecipitation, high ATP-sensitivities, elevation of Mg2+-ATPase activity, and stabilization of Ca2+-ATPase activity. 4) From these results, it is concluded that the biological activities of actins from the smooth adductor muscles of surf-clam and scallop are essentially the same as those of actins from the striated adductor muscles of scallop and the skeletal muscles of rabbit.