AGE-RELATED CHANGES IN PLOIDY CLASSES OF LIVER CELLS IN HUMAN AND RATS AS STUDIED BY THE FEULGEN-DNA CYTOFLUOROMETRY

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Using Feulgen-DNA cytofluorometry, we investigated quantitatively nuclear DNA content of human liver cells and rat liver cells in their aging processes, and compared the age-related changes in ploidy classes of liver cells between human and rats.Autopsy or biopsy specimens obtained from 20 patients (age ranging from 7 to 104 years old) with normal liver histology and function tests, were treated to make smear preparations of the single cells. Each nuclear DNA content of liver cells was determined by the Feulgen-DNA cytofluorometry using an automatic digital cytofluorometer (Nikon SPM-RFl). The ploidy classes of the liver cells were analyzed, with regard to binuclearity, on the computer-drawn graphs of the nuclear DNA content distribution. Rat liver cells (Donryu strain, age ranging from 21 to 730 days of life) were also similarly treated and analyzed.The cytofluorometric measurements on human liver cells revealed that about 90% of the cells are mononuclear diploid (MD) in adults of up to approximately 50 years of age and that, with further advancement of the age, the fraction of this ploidy class drops linearly with age, allowing the percentage of higher ploidy cells such as binuclear diploid (BD) and mononuclear tetraploid (MT) cells to increase steadily. At 104 years of age nearly 50% of the total liver cells are higher ploidy cells. On the other hand, in rat liver cells, 80% of the cells were MD at 3 weeks of life, but at 5 weeks the fraction of this ploidy class decreases rapidly while the fraction of BD cells increases reaching to nearly 40%. With advancement of animal age thereafter, the fraction of BD cells falls and MT cells becomes the majority, the percentage of which being 70% in adult rats with ages over 12 months.These results confirm the previous finding that polyploidization progresses steadily during the process of aging in both human and rats. As to the human liver cells, detailed standard curves were obtained in which age-related changes of the fraction of various ploidy classes were shown. The present study has also demonstrated that there are significant differences in the extent and the timing of polyploidization of liver cells between human and rats.
財団法人日本消化器病学会の論文

AGE-RELATED CHANGES IN PLOIDY CLASSES OF LIVER CELLS IN HUMAN AND RATS AS STUDIED BY THE FEULGEN-DNA CYTOFLUOROMETRY

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