Immunoelectron microscopic study of intermediate filaments of hepatocytes in human liver using three kinds of cytokeratin antibodies.

概要

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Extracted human liver tissues were examined by immunoelectron microscopy to study the heterogeneity of intermediate filaments of hepatocytes. Frozen sections of liver tissues were extracted by Triton X-100 and nucleases. Immunoelectron microscopy was performed by using MA-902 (CK No. 8), RPN. 1160 (CK No. 18) and CAM5.2 (CK No. 8, 18 & 19). Transmission electron microscopy showed that the cell borders were well defined by meshworks of numerous IFs and the pericanalicular sheaths composed of IFs were identified as tube-like structures. A meshwork of IFs was seen in the cytoplasm. The diameter of each IF was around 10nm. Immunoelectron microscopy showed that IFs appeared to be strongly stained with antibody MA-902 or CAM5.2 compared with antibody RPN. 1160. Double label immunoelectron microscopy showed that some bundles of filaments were stained with both antibody MA-902 and RPN. 1160. These results suggested that IFs without staining by CK No. 18 were seen in normal human hepatocytes and some of the IFs were shown to contain both cytokeratin polypeptides in a single filament.
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