Role of intermediate filaments and desmosome in the formation of polarity of hepatocytes.

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Isolated rat hepatocytes were obtained by perfusion method and purified by centrifugation and filtration. Immunofluorescence microscopy and immunoelectron microscopy (IEM) were applied to observe the relationship between intermediate filaments (IFs) and desmosomes (DSs) in the non detergent-treated and the detergent-treated hepatocytes using monoclonal antibodies to cytokeratin (CK) and desmoplakin (DP). Immediately after seeding hepatocytes were showed in round shape and divided into single cells. Untill 12 hrs later bile canaliculi and DP were not clearly observed. 24 hrs later hepatocytes attached to the dish and spread in a polygonal shape showing straight contacts with contiguous cells. CK filament networks formed cavities intercellularly and DP was observed at the cell borders and around BC in dotted lines. IEM revealed that IFs forming a filamentous network were densely noted at the cell borders and around the cavities where a large number of gold particles labeling DP were seen. Our results indicate that DS associated with IFs plays an important role in the formation of polarity of hepatocytes including the formation of PS.
社団法人日本肝臓学会の論文