カツオ肝臓のNAD(P)加水分解酵素-I : NAD(P) nucleosidaseの単離精製

概要

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A mixture of the three hydrolases, NAD(P) nucleosidase, nucleotide pyrophosphatase, and alkaline phosphatase, participating in the first step of the NAD(P) decomposition, was separated from skipjack liver. By Sephadex G-100 gel filtration, NAD(P) nucleosidase was successfully isolated from the mixture. During the gel filtration, the enzyme was found to separate into one main and one minor fraction. Both enzymes were purified further by several types of column chromatography using DEAE- and CM-cellulose, DEAE-Sephadex A-25, and hydroxylapatite and also by Sephadex G-100 gel filtration. The enzyme from the main fraction was finally purified about 1, 200-fold. It was demonstrated by polyacrylamide and starch gel electrophoresis that this enzyme consisted of three components. From the minor fraction, an enzyme preparation was obtained which was essentially composed of several other components but still contaminated with some components of the main fraction.
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