Involvement of Stathmin in Proliferation and Differentiation of Immortalized Human Endometrial Stromal Cells
スポンサーリンク
概要
- 論文の詳細を見る
Uterine endometrial stromal cells differentiate into decidual cells during the late secretory phase of the menstrual cycle and pregnancy. However, the biochemical mechanisms of decidualization have yet to be definitively elucidated. In the present study, we transfected primary human endometrial stromal cell with a temperature-sensitive mutant of simian virus 40 large T antigen and thereby established an immortalized stromal cell line (EtsT) in order to examine the role of stathmin, a cytosolic phosphoprotein that regulates microtubule dynamics, in stromal cell differentiation. When treated with the decidual stimulus dibutyryl-cAMP (db-cAMP) or forskolin, the fibroblastic cell-shaped EtsT cells transformed into large- and round-shaped cells and secreted large amounts of the decidual markers prolactin (PRL) and insulin-like growth factor binding protein-1 (IGFBP-1). Analysis of the stathmin protein levels in the db-cAMP- and forskolin-treated EtsT cells revealed that the total and phosphorylated protein levels dropped as decidualization progressed. Suppression of stathmin expression by transfection with small interfering RNA (siRNA) suppressed EtsT cell proliferation. It also abolished db-cAMP-induced PRL and IGFBP-1 mRNA expression and protein secretion. Thus, stathmin expression can be considered an integral factor regulating the initial stage of the process of human endometrial stromal cell differentiation.
- 日本繁殖生物学会の論文
著者
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Isaka Keiichi
Department Of Obstetrics And Gynaecology Tokyo Medical University Hospital
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Kogo Hiroshi
Department Of Anatomy And Cell Biology Gunma University School Of Medicine
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YOSHIE Mikihiro
Department of Pharmacology, Tokyo University of Pharmacy and Life Science
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Tamura Kazuhiro
Department Of Pharmacology Tokyo University Of Pharmacy And Life Science
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Tamura Kazuhiro
Department Of Endocrine Pharmacology Tokyo University Of Pharmacy And Life Science
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HARA Takahiko
Stem Cell Project Group, The Tokyo Metropolitan Institute of Medical Science
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Hara Takahiko
Stem Cell Project Group The Tokyo Metropolitan Institute Of Medical Science Tokyo Metropolitan Organ
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Tamura Kazuhiro
Department of Endocrine Pharmacology, Tokyo University of Pharmacy and Life Science
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YOSHIE Mikihiro
Department of Endocrine Pharmacology, Tokyo University of Pharmacy and Life Science
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