Effect of Thyroid Hormone on CYP19 Expression in Ovarian Granulosa Cells From Gonadotropin-Treated Immature Rats
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概要
- 論文の詳細を見る
The present study was designed to determine the mechanisms by which serum estradiol levels are enhanced during gonadotropin-induced ovarian development in hypothyroid immature rats. Thyroidectomized (Tx)-immature rats receiving thyroidectomy on day 22 were treated with 8 IU equine chorionic gonadotropin (eCG) at 26 days of age to induce follicular development in ovaries. Ovaries or ovarian granulosa cells were collected on the first proestrus (day 28). Enhanced expression of aromatase (CYP19) mRNA induced by eCG treatment was inhibited by injection of thyroxine (T4: 5 μg × 6 times) in Tx rats. Ovarian granulosa cells harvested from Tx rats were cultured for 24 h in chemically defined serum-free medium containing gonadotropin in the presence of 3,5,3-triidothyronine (T3). Addition of low doses of T3 (10−8, 10−7 M) to the medium significantly decreased 17β-estradiol (E2) levels, whereas high doses of T3 (10−6, 10−5 M) did not inhibit the levels. Similar effects of T3 on CYP19 mRNA expression were observed. These results indicate that augmentation of E2 production in hypothyroid animals may be mediated, in part, via the stimulation of CYP19 mRNA expression in granulosa cells, which is suppressed by thyroid hormone.
- 社団法人 日本薬理学会の論文
- 2004-04-01
著者
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Kogo Hiroshi
Department Of Anatomy And Cell Biology Gunma University School Of Medicine
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Toda Katsumi
Department Of Medical Chemistry Kochi Medical School
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Toda Katsumi
Department Of Molecular Genetics School Of Medicine Kochi University
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Tamura K
Department Of Endocrine Pharmacology School Of Pharmacy Tokyo University Of Pharmacy & Life Scie
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Shimizu Yoko
Department Of Endocrine Pharmacology School Of Pharmacy Tokyo University Of Pharmacy & Life Scie
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Tamura Kazuhiro
Department Of Endocrine Pharmacology Tokyo University Of Pharmacy And Life Science
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HATSUTA Minoru
Department of Endocrine Pharmacology, School of Pharmacy, Tokyo University of Pharmacy & Life Scienc
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Kogo Hiroshi
Department Of Endocrine Pharmacology School Of Pharmacy Tokyo University Of Pharmacy & Life Scie
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Hatsuta Minoru
Department Of Endocrine Pharmacology School Of Pharmacy Tokyo University Of Pharmacy & Life Scie
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