Different-batch metabolome analysis of Saccharomyces cerevisiae based on gas chromatography/mass spectrometry(METHODS)
スポンサーリンク
概要
- 論文の詳細を見る
Each experimental step in metabolomics based on mass spectrometry for microorganisms, such as cultivation, sampling, extraction of metabolites, analysis, and data processing includes different systematic errors. Even if the same protocol is used, it is difficult to compare the data from different cultivation days or different analysis days. To obtain reliable quantitative data, it is necessary to develop an analytical workflow that can reduce errors from different batch of cultivation and analysis days. We compared metabolomics methods for Saccharomyces cerevisiae in terms of reproducibility to optimize the analytical workflow, particularly quenching and data processing. Our data also showed that reproducible data could be obtained with high signal to noise ratio. Therefore, we optimized a time segmented selective ion monitoring (SIM) method for high sensitive analysis with low-risk of false positives. The optimized workflow was applied to metabolome analysis of single transcription factor deletion mutants. As a result, we obtained clusters that were independent of cultivation day and analysis day but were strain-dependent. This study can help to implement large-scale or long-term studies, in which samples are divided among several laboratories because of the high number of samples.
- 公益社団法人日本生物工学会の論文
著者
-
Bamba Takeshi
Department Of Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univers
-
KAWASE Naoki
Department of Agricultural Chemistry, Kyoto University
-
Fukusaki Eiichiro
Department Of Biotechnology Faculty Of Engineering Osaka University
-
Tsugawa Hiroshi
Department Of Biotechnology Graduate School Of Engineering Osaka University
-
Kawase Naoki
Department of Biotechnology, Graduate School of Engineering, Osaka University
関連論文
- Pyrolysis GC-MS-based metabolite fingerprinting for quality evaluation of commercial Angelica acutiloba roots(METHODS)
- 超臨界流体クロマトグラフィー/質量分析による多様な脂質のハイスループット網羅的分析(生物工学論文賞)
- 2Mp21 ゴマ由来ピペリトール8セサミン合成酵素CYP81Q1を発現させたシロイヌナズナに含まれるリグナン関連化合物のプロファイリング(生合成・天然物化学,一般講演)
- Size Exclusion Chromatography of Standard Polystyrenes with a Wide Range of Molecular Weight Up to 7.45 × 10^6 on Monolithic Silica Capillary Columns
- Production of Eucommia-rubber from Eucommia ulmoides Oliv. (Hardy Rubber Tree)
- DrEFTIR : The data mining software for fourier transform near-infrared reflectance spectroscopy focused on food metabolic finger printing
- Characterization of the C-terminal truncated form of amylopullulanase from Lactobacillus plantarum L137(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- メタボロミクスの技術をベースとしたマルチマーカープロファイリング--緑茶官能評価への応用
- 2Ia03 トチュウバイオマスからのトランス型ポリイソプレン生産技術の開発(バイオマス・資源・エネルギー工学,一般講演)
- 2Gp11 超臨界流体クロマトグラフィー/質量分析を用いた脂質プロファイリングにおける誘導体化法の検討(代謝工学,一般講演)
- An approach to peak detection in GC-MS chromatograms and application of KNApSAcK database in prediction of candidate metabolites
- 2Ep24 トランスポリイソプレン生合成のトランスクリプトーム解析(植物細胞工学・組織培養・育種工学,一般講演)
- Influences of biofluid sample collection and handling procedures on GC-MS based metabolomic studies(METHODS)
- メタボロミクスにおける超臨界流体クロマトグラフィーの可能性(生物工学における超臨界流体テクノロジーの可能性)
- ポストトランスクリプトミクス研究の最前線(第61回大会シンポジウム報告)
- Characterization of Gene Encoding Amylopullulanase from Plant-Originated Lactic Acid Bacterium, Lactobacillus plantarum L137(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- 食の機能・安全を科学する : おいしさから生理活性,安全性の評価まで(第62回大会シンポジウム報告)
- 2P-1134 超臨界流体クロマトグラフィーを用いたベータクリプトキサンチンエステル分析技術の開発(3cオミクス解析,一般講演,代謝生理学・発酵生産,伝統の技と先端科学技術の融合)
- 2P-1133 超臨界流体クロマトグラフィーにおける分離特性の解析 : カラム修飾が分子認識能に与える影響について(3cオミクス解析,一般講演,代謝生理学・発酵生産,伝統の技と先端科学技術の融合)
- 2Ep26 トチュウのトランス型ポリイソプレン生合成系関連遺伝子の特定と発現(植物細胞工学・組織培養・育種工学,一般講演)
- 2Ep25 タバコを用いたトチュウ(Eucommia uhmoides Oliver)由来トランスポリイソプレン合成酵素の機能解析(植物細胞工学・組織培養・育種工学,一般講演)
- Removal of Phenolic Endocrine Disrupters by Portulaca oleracea(ENVIRONMENTAL BIOTECHNOLOGY)
- 2P-1131 安定同位体希釈率の経時変化情報による中央代謝ターンオーバー解析(3cオミクス解析,一般講演,代謝生理学・発酵生産,伝統の技と先端科学技術の融合)
- 2Bp06 メタノール資化酵母の脂肪酸不飽和化酵素遺伝子の単離(遺伝子工学,一般講演)
- 2Gp13 メタボローム解析から捉えた線虫Caenorhabditis elegansのlife cycle(代謝工学,一般講演)
- 2Gp10 LC-ESI-IT-TOF-MSを用いた定量的micro RNA解析手法の開発(代謝工学,一般講演)
- Biosynthetic Pathway for the C45 Polyprenol, Solanesol, in Tobacco
- Comparative analysis of a CFo ATP synthase subunit II homologue derived from marine and fresh-water algae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Isolation and characterization of a novel antistress gene from Chlamydomonas sp. W80(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Transient Gene Expression in Guard Cell Chloroplasts of Tobacco Using ArF Excimer Laser Microablation(PLANT BIOTECHNOLOGY)
- Expression and Purification of Recombinant Human Annexin A2 in Pichia pastoris and Utility of Expression Product for Detecting Annexin A2 Antibody(MEDICAL BIOTECHNOLOGY)
- Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus: a Dethiobiotin producing Bacterium
- Comprehensive metabolite profiling of phyA phyB phyC triple mutants to reveal their associated metabolic phenotype in rice leaves(PLANT BIOTECHNOLOGY)
- High Throughput and Exhaustive Analysis of Diverse Lipids by Using Supercritical Fluid Chromatography-Mass Spectrometry for Metabolomics(METHODS)
- Quantitative Analysis of Anionic Metabolites for Cathamnthus roseus by Capillary Electrophoresis Using Sulfonated Capillary Coupled with Electrospray lonization-Tandem Mass Spectrometry(METHODS)
- Single Cell-Based Analysis of Torenia Petal Pigments by a Combination of ArF Excimer Laser Micro Sampling and Nano-High Performance Liquid Chromatography (HPLC)-Mass Spectrometry(METHODS)
- Pressure-Assisted Capillary Electrophoresis Mass Spectrometry Using Combination of Polarity Reversion and Electroosmotic Flow for Metabolomics Anion Analysis(METHODS)
- Stable Isotope Dilution-Based Accurate Comparative Quantification of Nitrogen-Containing Metabolites in Arabidopsis thaliana T87 Cells Using in Vivo ^N-Isotope Enrichment
- An Isotope Effect on the Comparative Quantification of Flavonoids by Means of Methylation-Based Stable Isotope Dilution Coupled with Capillary Liquid Chromatography/Mass Spectrometry(Methods)
- Novel plant transformation system by gene-coated gold particle introduction into specific cell using ArF excimer laser
- Plant Metabolomics : Potential for Practical Operation
- 421 ISOLATION AND CHARACTERIZATION OF BIO-ACTIVE COMPOUNDS PRODUCED BY THE CO-CULTURE OF A CALLUS AND A FUNGUS
- Synthesis of the Four Possible Stereoisomers of 21-Methyl-8-Pentatriacontene, the Female Contact Sex Pheromone of the Yellow-Spotted Longicorn Beetle, Psacothea hilaris
- A High-Throughput and Solvent-free Method for Measurement of Natural Polyisoprene Content in Leaves by Fourier Transform Near Infrared Spectroscopy(METHODS)
- Metabolic Profiling of Angelica acutiloba Roots Utilizing Gas Chromatography-Time-of-Flight-Mass Spectrometry for Quality Assessment Based on Cultivation Area and Cultivar via Multivariate Pattern Recognition(BREWING AND FOOD TECHNOLOGY)
- Quantification of trans-1,4-Polyisoprene in Eucommia ulmoides by Fourier Transform Infrared Spectroscopy and Pyrolysis-Gas Chromatography/Mass Spectrometry(METHODS)
- 1J10-4 ^1H NMR BASED METABOLIC PROFILING IN THE QUALITY EVALUATION OF JAPANESE GREEN TEA
- Phytochelatins inhibit the metal-induced aggregation of α-crystallin(PLANT BIOTECHNOLOGY)
- Synthesis of Anti-Tumor Dimeric Indole Alkaloids in Catharanthus roseus Was Promoted by Irradiation with Near-Ultraviolet Light at Low Temperature
- Cloning and Characterization of Mevalonate Pathway Genes in a Natural Rubber Producing Plant, Hevea brasiliensis
- High-Throughput and Highly Sensitive Analysis Method for Polyisoprene in Plants by Pyrolysis-Gas Chromatography/Mass Spectrometry
- Molecular Cloning and Partial Characterization of a Peroxidase Gene Expressed in the Roots of Portulaca oleracea cv., One Potentially Useful in the Remediation of Phenolic Pollutants
- 超臨界流体クロマトグラフィー/質量分析を用いたメタボリックプロファイリングの食品科学への応用(食の機能・安全を科学する-おいしさから生理活性,安全性の評価まで)
- Application of Fourier transform near-infrared spectroscopy to optimization of green tea steaming process conditions(BREWING AND FOOD TECHNOLOGY)
- Predication of Japanese green tea (Sen-cha) ranking by volatile profiling using gas chromatography mass spectrometry and multivariate analysis(BREWING AND FOOD TECHNOLOGY)
- Practical non-targeted gas chromatography/mass spectrometry-based metabolomics platform for metabolic phenotype analysis(BIOINFORMATICS)
- メタボリックプロファイリングによる食品/生薬の品質評価
- 1Lp04 LC/MS/MSに基づくメタボローム解析におけるMSn情報を用いた代謝物群判別法の開発(オミクス解析/生体情報工学・バイオインフォマティクス,一般講演)
- Application of supercritical fluid chromatography/mass spectrometry to lipid profiling of soybean(METHODS)
- Application of electrospray ionization ion trap/time-of-flight mass spectrometry for chemically-synthesized small RNAs(METHODS)
- Metabolomics-based component profiling of hard and semi-hard natural cheeses with gas chromatography/time-of-flight-mass spectrometry, and its application to sensory predictive modeling(BREWING AND FOOD TECHNOLOGY)
- Metabolic profiling and identification of the genetic varieties and agricultural origin of Cnidium officinale and Ligusticum chuanxiong(PLANT BIOTECHNOLOGY)
- Highly sensitive and rapid profiling method for carotenoids and their epoxidized products using supercritical fluid chromatography coupled with electrospray ionization-triple quadrupole mass spectrometry(METHODS)
- Identification of Biomarkers of Stent Restenosis With Serum Metabolomic Profiling Using Gas Chromatography/Mass Spectrometry
- Gas chromatography-mass spectrometry based metabolic profiling for the identification of discrimination markers of Angelicae Radix and its application to gas chromatography-flame ionization detector system(METHODS)
- Characterization of Bisphenol A Metabolites Produced by Portulaca oleracea cv. by Liquid Chromatography Coupled with Tandem Mass Spectrometry
- Metabolite profiling of soy sauce using gas chromatography with time-of-flight mass spectrometry and analysis of correlation with quantitative descriptive analysis(BREWING AND FOOD TECHNOLOGY)
- Gallic acid oxidizes Met residues in peptides released from bovine β-lactoglobulin by in vitro digestion(BREWING AND FOOD TECHNOLOGY)
- A High-Throughput and Solvent-free Method for Measurement of Natural Polyisoprene Content in Leaves by Fourier Transform Near Infrared Spectroscopy
- Metabolomics-based component profiling of hard and semi-hard natural cheeses with gas chromatography/time-of-flight-mass spectrometry, and its application to sensory predictive modeling
- Metabolic fingerprinting of hard and semi-hard natural cheeses using gas chromatography with flame ionization detector for practical sensory prediction modeling(BREWING AND FOOD TECHNOLOGY)
- Highly sensitive and rapid profiling method for carotenoids and their epoxidized products using supercritical fluid chromatography coupled with electrospray ionization-triple quadrupole mass spectrometry
- Application of ion mobility-mass spectrometry to microRNA analysis(METHODS)
- Effect of gallic acid on peptides released by trypsin digestion of bovine α-casein(BREWING AND FOOD TECHNOLOGY)
- ガスクロマトグラフィー質量分析を用いた代謝産物表現型解析のための実用的なノンターゲットメタボロミクス解析プラットホーム(2012論文賞)
- 3Ga09 超臨界流体クロマトグラフ-三連四重極型質量分析計を用いたハイスループット脂質メタボロミクスシステムの開発(オミクス解析,一般講演)
- 3Gp03 超臨界流体クロマトグラフィー質量分析計を用いた食用油中の3-MCPD脂肪酸エステル類の高感度迅速分析法の開発(オミクス解析/生合成,天然物化学/環境工学,廃水処理技術,一般講演)
- 3Ga12 超臨界流体クロマトグラフィー/質量分析を用いた残留農薬迅速一斉分析方法の開発(オミクス解析,一般講演)
- 3Ga11 ガスクロマトグラフィー/質量分析を用いた揮発性酸化脂質の分析(オミクス解析,一般講演)
- 3Ga10 超臨界流体クロマトグラフィー/質量分析を用いた赤血球脂質のプロファイリング(オミクス解析,一般講演)
- 3Ga08 超臨界流体クロマトグラフィー/質量分析を用いた酸化リン脂質異性体分析系の構築(オミクス解析,一般講演)
- 3Gp02 高温適応酵母のオミクス解析による耐熱性獲得機構の考察(オミクス解析/生合成,天然物化学/環境工学,廃水処理技術,一般講演)
- Metabolic Profiling of Oxidized Lipid-Derived Volatiles in Blood by Gas Chromatography/Mass Spectrometry with In-Tube Extraction
- Metabolite profiling of soy sauce using gas chromatography with time-of-flight mass spectrometry and analysis of correlation with quantitative descriptive analysis
- Gas chromatography-mass spectrometry based metabolic profiling for the identification of discrimination markers of Angelicae Radix and its application to gas chromatography-flame ionization detector system
- Gallic acid oxidizes Met residues in peptides released from bovine β-lactoglobulin by in vitro digestion
- 3P-026 内分泌かく乱作用の評価に資するGC/MSを用いたステロイド類の一斉分析系の構築
- 3P-025 メタボローム情報による回帰モデル構築のためのサンプル選択アルゴリズムの開発
- 3P-029 GC-MSを用いたメタボロミクスにおけるキャリヤーガス種の検討とライブラリー構築
- 3P-024 ブレオマイシン誘発肺線維症モデルラットの肺サーファクタントを対象とした脂質メタボローム解析
- 3P-203 水素細菌Ralstonia eutrophaの従属栄養条件下における炭酸固定経路の機能解析(バイオプロセス,一般講演)
- 3P-023 超臨界クロマトグラフィー質量分析を用いたハイスループット農薬一斉分析法の開発
- 3P-030 glucose誘導性の分解を受けにくいalpha-glucoside transporter の発現が、酵母にもたらす増殖阻害
- 3P-018 メタボロミクス技術に基づく1 : プロパノール生産組換え大腸菌の遺伝子改変戦略の構築
- 3P-027 ガスクロマトグラフィ-質量分析とOPLSモデリングを用いたメタボロミクスを基盤とした出芽酵母1-ブタノール耐性関連遺伝子の半合理的特定
- 3P-028 イオントラップ-飛行時間型質量分析計を用いた抱合体の同定方法の開発
- 3P-021 定量的メタボローム解析に資する新規相対定量法
- 3P-020 ストレス条件下のメタボローム解析による出芽酵母のエタノール耐性予測
- Highly sensitive and selective analysis of widely targeted metabolomics using gas chromatography/triple-quadrupole mass spectrometry(METHODS)
- Different-batch metabolome analysis of Saccharomyces cerevisiae based on gas chromatography/mass spectrometry(METHODS)