Highly sensitive and selective analysis of widely targeted metabolomics using gas chromatography/triple-quadrupole mass spectrometry(METHODS)
スポンサーリンク
概要
- 論文の詳細を見る
In metabolomics studies, gas chromatography coupled with time-of-flight or quadrupole mass spectrometry has frequently been used for the non-targeted analysis of hydrophilic metabolites. However, because the analytical platform employs the deconvolution method to extract single-metabolite information from co-eluted peaks and background noise, the extracted peak is artificial product depending on the mathematical parameters and is not completely compatible with the pure component obtained by analyzing a standard compound. Moreover, it has insufficient ability for quantitative metabolomics. Therefore, highly sensitive and selective methods capable of pure peak extraction without any complicated mathematical techniques are needed. For this purpose, we have developed a novel analytical method using gas chromatography coupled with triple-quadrupole mass spectrometry (GC-QqQ/MS). We developed a selected reaction monitoring (SRM) method to analyze the trimethylsilyl derivatives of 110 metabolites, using electron ionization. This methodology enables us to utilize two complementary techniques-non-targeted and widely targeted metabolomics in the same sample preparation protocol, which would facilitate the formulation or verification of novel hypotheses in biological sciences. The GC-QqQ/MS analysis can accurately identify a metabolite using multichannel SRM transitions and intensity ratios in the analysis of living organisms. In addition, our methodology offers a wide dynamic range, high sensitivity, and highly reproducible metabolite profiles, which will contribute to the biomarker discoveries and quality evaluations in biology, medicine, and food sciences.
著者
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Bamba Takeshi
Department Of Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univers
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Nishiumi Shin
Division Of Applied Chemistry In Bioscience Department Of Agrobioscience Graduate School Of Agricult
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Fukusaki Eiichiro
Department Of Biotechnology Faculty Of Engineering Osaka University
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SAKUI Norihiro
Agilent Technologies
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Tsujimoto Yuki
Department Of Cardiology Kurashiki Central Hospital
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Tsugawa Hiroshi
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Sugitate Kuniyo
Agilent Technologies Japan Ltd.
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Sakui Norihiro
Agilent Technologies Japan, Ltd.
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Nishiumi Shin
Division of Gastroenterology, Kobe University Graduate School of Medicine
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Tsujimoto Yuki
Department of Biotechnology, Graduate School of Engineering, Osaka University
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