カラムスイッチング-オンカラム蛍光誘導体化LC法による鶏肉中コリスチンの分析

概要

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A simple, rapid, and precise method was developed for the determination of colistin [CL: mixture of colistin-A (CLA) and colistin-B (CLB)] in chicken tissues by LC/FL using an on-column fluorescence derivatization and column-switching technique. CL in chicken tissues was extracted with 10% trichloroacetic acid aqueous solution and the crude extract was purified on an Oasis^[○!R] HLB cartridge. CLA and CLB were derivatized into their respective o-phthalaldehyde fluorophores by an on-column reaction and then subjected to column-switching and subsequent chromatographic separation on a reversed-phase C_<18> column. The on-column fluorescence derivatization LC method was superior to conventional pre-column fluorescence derivatization in term of the stability of the reaction products. The limits of detection (S/N=3) of CLA and CLB were 0.01μg/mL and 0.003μg/mL, respectively. The limits of quantification (S/N>10) of CLA and CLB were 0.04>g/mL and 0.01>g/mL, respectively. The average recoveries of CLA and CLB from chicken meat ranged from 67.9% to 78.9% and from 65.9% to 78.1%, respectively. The results suggest that the oncolumn fluorescence derivatization LC method is useful for the determination of CL in chicken tissues.
2013-12-24