The Behavior of _L-Ascorbic Acid in the Prolyl 4-Hydroxylase Reaction(Food & Nutrition)
スポンサーリンク
概要
- 論文の詳細を見る
_L-Ascorbic acid (AsA) is specifically required for the decarboxylation of α-ketoglutarate (KGA) in the prolyl 4-hydroxylase reaction, due to its structural characteristics. For further clarification of the role of AsA in proline hydroxylation, AsA and its oxidation products during proline hydroxylation were determined by high-performance liquid chromatography (HPLC) using a radioisotope analyzer as a detection system. AsA was oxidized in the uncoupled reaction system without a suitable peptide substrate or with an unsuitable one such as poly-L-proline. Furthermore, the oxidation of AsA occurred in the complete reaction, even in the presence of a high concentration of dithiothreitol (DTT) in the reaction solution. These results indicated that the uncoupled reaction inevitably occurred during proline hydroxylation and this uncoupled reaction was accompanied by the oxidation of AsA, which led to its consumption. The reaction pathway of this hydroxylation was also discussed.
- 社団法人日本農芸化学会の論文
- 1988-03-23
著者
-
Kurata T
Faculty Of Applied Life Sciences Niigata University Of Pharmacy And Applied Life Sciences
-
Kurata Tadao
Department Of Food And Nutrition Ochanomizu University
-
Kurata Tadao
Department Of Applied Sciences Niigata University Of Pharmacy And Applied Sciences
-
ARAKAWA Nobuhiko
Department of Nutrition & Food Science, Ochanomizu University
-
YU Rina
Department of Food and Nutrition, Ochanomizu University
-
Yu Rina
Department Of Food And Nutrition Ochanomizu University
-
Arakawa N
Department Of Nutrition And Food Science Ochanomizu University
-
ARAKAWA Nobuhiko
Department fo Nutrition and Food Science, Ochanomizu University
関連論文
- Influence of Cigarette Smoke on the L-Ascorbic Acid Metabolism and the Activities of Drug-Metabolizing Enzyme in Rats
- Physiological Role of L-Ascorbic Acid in Rats Exposed to Cigarette Smoke
- Glutathione Reacts with Glyoxal at the N-Terminal
- Contribution of a High Dose of L-Ascorbic Acid to Carnitine Synthesis in Guinea Pigs Fed High-Fat Diets
- Effects of L-Ascorbic Acid on Lysyl Oxidase in the Formation of Collagen Cross-links(Food & Nutrition Science)
- Stabilization of L-Ascorbic Acid by Superoxide Dismutase and Catalase
- Formation Mechanism of Monodehydro-L-ascorbic Acid and Superoxide Anion in the Autoxidation of L-Ascorbic Acid
- Effects of Thiol Compounds on the Formation of Nitriles from Glucosinolates(Food & Nutrition)
- Effects of pH and Ferrous Ion on the Degradation of Glucosinolates by Myrosinase(Food & Nutrition)
- Chemical characteristics of dehydro-L-ascorbic acid
- Time-Dependent Changes of Tissue Erythorbic Acid Concentrations in Guinea Pigs
- Autoxidation Reaction Mechanism for L-Ascorbic Acid-related Compounds in Methanol without Metal Ion Catalysis
- Contents of Erythorbic Acid in the Tissues of Guinea Pigs Intraperitoneally Administered Erythorbic Acid
- Autoxidation Reaction Mechanism for L-Ascorbic Acid in Methanol without Metal Ion Catalysis
- Formation of L-Threonolactone and Oxalic Acid in the Autoxidation Reaction of L-Ascorbic Acid : Possible Involvement of Singlet Oxygen
- The Behavior of _L-Ascorbic Acid in the Prolyl 4-Hydroxylase Reaction(Food & Nutrition)
- The Role of _L-Ascorbic Acid in the Decarboxylation of α-Ketoglutarate Catalyzed by Prolyl 4-Hydroxylase(Food & Nutrition)
- Study on the Volatile Compounds of Fish Sauces : Shottsuru, Nampla and Noucman(Food & Nutrition)
- Scavenging Effects of L-Ascorbic Acid on 1, 1-Diphenyl-2-picrylhydrazyl in Homogeneous and Heterogeneous Reaction Systems
- Regulation of Cigarette Smoke-Induced Cytochrome P4501A1 Gene Expression in Osteogenic Disorder Shionogi Rat Liver and in Lung by Large Ascorbic Acid Dose(Biochemistry & Molecular Biology)
- Hepatic Branched-Chain α-Keto Acid Dehydrogenase Complex in Female Rats : Activation by Exercise and Starvation
- Glutathione Reacts with Glyoxal at the N-Terminal
- Capsaicin Produce Apoptosis in Korean Stomach Cancer Cell Line, SNU-1
- The Effect of L-Ascorbic Acid on Age-Related Changes of Pyridinoline in Cartilage Collagen of Guinea Pigs
- Contents of Erythorbic Acid in the Tissues of Guinea Pigs Intraperitoneally Administered Erythorbic Acid
- Effect of High Concentration of Ascorbate on Catalase Activity in Cultured Cells and Tissues of Guinea Pigs
- A Positive Correlation between Catalase Activity and Ascorbate Uptake in the Tissues of Guinea Pigs and Cultured Cells of Mammals
- Activities of D and L-Xyloascorbic Acid and D and L-Araboascorbic Acid as a Cofactor for Dopamine β-Hydroxylase Reaction
- The Level of Orally Ingested Vitamin C Affected the Expression of Vitamin C Transporters and Vitamin C Accumulation in the Livers of ODS Rats
- Role of Hydrogen Peroxide in the Inhibitory Effect of Ascorbate on Cell Growth.
- The Regulatory Effect of Ascorbate on the Carmnitine Synthesis in Primary Cultured Guinea Pig Hepatocytes.
- Age-Related Changes in the Pyridinoline Content of Guinea Pigs Cartilage and Achilles Tendon Collagen.
- An Improved High-Performance Liquid Chromatographic Assay for the Determination of Pyridinoline in Connective Tissues.
- The Behavior of L-Ascorbic Acid in the Healing Process of Dorsal Wounds in Guinea Pigs.
- Comparison of Absorption of Erythorbic Acid and Ascorbic Acid in Guinea Pig Small Intestine.
- Vitamin C Activity of Erythorbic Acid in Ascorbic Acid-Deficient Guinea Pigs.
- Vitamin C Activity of 2-O-.ALPHA.-D-Glucopyranosyl-L-Ascorbic Acid in Guinea Pigs.
- Effect of Fasting on the Endogeneous Inhibitor of Calcium Activated Factor in the Skeletal Muscle of Rats
- A Rapid and Sensitive Method for the Determination of Ascorbic Acid using 4, 7-Diphenyl-l, 10- phenanthroline
- Antimicrobial Activity of Kumazasa (Sasa albo-marginata)
- Some Aspects of the Role of Ascorbic Acid in Proline Hydroxylation
- Isolation and Characterization of an Intermediate Product in the Degradation of 2.3-Diketo-L-gulonic Acid
- Mutagen Formation after Heating of Mixtures of Guanidino Compounds, Amino Acids and Glucose.
- Effect of Fasting on Calcium Activated Protease in Rat Skeletal Muscle