Activities of D and L-Xyloascorbic Acid and D and L-Araboascorbic Acid as a Cofactor for Dopamine β-Hydroxylase Reaction
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概要
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L-Xyloascorbic acid (L-xylo-ASA) and its three stereoiso-mers, D-xyloascorbic acid (D-xylo-AsA), L-araboascorbic acid (L-arabo-AsA) and D-araboascorbic acid (D-arabo-AsA), have been considered to show some differences in vitamin C activity. In this paper the effect of L-xylo-AsA, D-xylo-AsA, L-arabo-AsA and D-arabo-AsA on the activity of dopamine β-hydroxylase was studied to clarify whether or not the struc-tural specificities of these stereoisomers have different effects on enzyme activity. The maximum velocity (Vmax) of the hydroxylation and Km for ascorbic acid were calculated using double-reciprocal plotting. Vmax for L-xylo-AsA was estimated to be 201 nmol/min/mg protein and those of D-xylo-AsA, L-arabo-AsA and D-arabo-AsA were 157 nmol/min/mg pro-tein, 112 nmol/min/mg protein and 194 nmol/min/mg protein, respectively. Km for L-xylo-AsA was 1.5 mM and those for D-xylo-AsA, L-arabo-AsA and D-arabo-AsA were 2.3 mM, 2.7 mM and 1.4 mM, respectively. The effect of D-arabo-AsA on the activity of dopamine β-hydroxylase was almost the same as that of L-xylo-AsA, while D-xylo-AsA and L-arabo-AsA showed smaller effects. Our results suggest that the configuration at carbon 4 might be more important than that of the hydroxyl group at carbon 5 for the development of the activity as a cofactor for dopamine β-hydroxylase reaction.
- 財団法人 学会誌刊行センターの論文
著者
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Kurata T
Faculty Of Applied Life Sciences Niigata University Of Pharmacy And Applied Life Sciences
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Kurata Tadao
Institute Of Environmental Science For Human Life Ochanomizu University
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ARAKAWA Nobuhiko
Department of Nutrition & Food Science, Ochanomizu University
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Mori Miki
Department Of Applied Chemistry Hiroshima University
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Suzuki Emiko
Faculty Of Life Science Ochanomizu University
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Suzuki Emiko
Department Of Human Biological Studies Ochanomizu University
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Kurata Tadao
Niigata Univ. Pharmacy And Applied Sci. Niitsu Jpn
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SHIBATA Miho
Department of Nutrition and Food Science, Ochanomizu University
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SUZUKI Emiko
Department of Human Biological Studies
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KURATA Tadao
Institute of Environmental Science for Human Life
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ARAKAWA Nobuhiko
Department fo Nutrition and Food Science, Ochanomizu University
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SUZUKI Emiko
Department of Food and Nutrition, Ochanomizu University
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