Exon skipping of exonuclease 1 in MRL/MpJ mice is caused by a nucleotide substitution of the branchpoint sequence in intron eight
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概要
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In MRL/MpJ mice, there is a genetic mutation of exonuclease 1 (Exo1), in which the exon 9 is sometimes deleted. In the presentstudy, to check the generation ofthe spliced exons, exon 8-intron 8-exon 9 (pCX/Ex/EIE/B and pCX/Ex/EIE/M) plasmids were temporally transfected in vitro into BALB 3T3 cells, and RT-PCR using appropriate primer pair was carried out 1 day after transfection. In these constructions, pCX/Ex/EIE/B was derived from genomic sequence of C57BL/6 mice, and pCX/Ex/EIE/M was from MRL/MpJ. A spliced band was detected in pCX/Ex/EIE/B, but was present little or very weakly in pCX/Ex/EIE/M. Next, the same spliced band was demonstrated in the pCX/Ex/EIE/M(T) plasmid, in which the branchpoint sequence (BPS) of pCX/Ex/EIE/M including the exon 9 was changed into that of pCX/Ex/EIE/B. The splicing did not occur in the del1/B mutant, in which 1960 nucleotides of the intron 8 were deleted, whereas it was detected in the del2/B plasmid deleted 1036 nucleotides in its middle region. These results suggest that the nucleotide T to A mutation of the BPS in the intron 8 is at least a sufficient for generation of splice variants (tr-1 and tr-2 Exo1).
- 北海道大学の論文
- 2004-11-30
著者
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KON Yasuhiro
Laboratory of Experimental Animal Science, Graduate School of Veterinary Medicine, Hokkaido Universi
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Kon Yasuhiro
Laboratory Of Anatomy Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokka
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ENDOH Daiji
Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University
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Endoh Daiji
Faculty Of Veterinary Medicine Hokkaido Univ.
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Agui Takashi
Laboratory Of Laboratory Animal Science And Medicine Department Of Disease Control Graduate School O
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Tachi Atsushi
Laboratory Of Public Health Graduate School Of Veterinary Medicine Hokkaido University
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SASAKI Nobuya
Laboratory of Experimental Animal Science, Graduate School of Veterinary Medicine, Hokkaido Universi
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Agui T
Laboratory Of Laboratory Animal Science And Medicine Department Of Disease Control Graduate School O
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Namiki Yuka
Laboratory of Experimental Animal Science, Graduate School of Veterinary Medicine, Hokkaido Universi
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Kon Yasuhiro
Laboratory Of Anatomy Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokka
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ENDOH Daiji
Department of Basic Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University
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NAMIKI Yuka
Laboratory of Anatomy, Graduate School of Veterinary Medicine, Hokkaido University
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