Effects of Antioxidants on Induction of Apoptosis in Bursal Cells of Fabricius during In Vitro Cultivation
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概要
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After physically disrupting cell contacts, apoptosis of bursal cells of Fabricius was induced during in vitro cultivation. The percentage of apoptotic cells increased with incubation time and approximately 70% cells represented apoptosis after 6 hr of incubation. The induction of apoptosis was significantly inhibited by treatment of the cells with ascorbic acid (vitamin C), but not with trolox, a vitamin E analog. An intense DNA ladder pattern was shown at 6 hr post-isolation, which is a biochemical hallmark of apoptosis. Treatment of the cells with ascorbic acid inhibited the DNA fragmentation, but trolox did not. To monitor the intracellular production of reactive oxygen species (ROSs), the intensity of fluorescence emitted from DCFH-DA was measured. The intensity of fluorescence from cells incubated for 0.5-2 hr was approximately 2-fold higher than that from cells at 0 hr. The relative intensity of fluorescence decreased immediately after the addition of ascorbic acid to the cells. The intensity from the cells treated with ascorbic acid was 20-30% of that from the control cells at each incubation time. For trolox, the intensity was 50-70% of that from the control cells at each 1 to 2 hr incubation time. When ROSs-induced lipid peroxidation was assessed using cis-parinaric acid (PnA) as a monitor molecule, lipid peroxidation was found to occur in the control cells after isolation of the bursal cells. Treatment of the cells with trolox reduced lipid peroxidation, but treatment with ascorbic acid enhanced peroxidation.
- 公益社団法人 日本獣医学会の論文
著者
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ITOH Haruo
Department of Electrical, Electronic and Computer Engineering, Chiba Institute of Technology
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Kanda Satoshi
Department Of Bioresource Development Hiroshima Prefectural University
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Arai Soichiro
Department Of Experimental Radiobiology Faculty Of Veterinary Medicine Hokkaido University
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ENDOH Daiji
Department of Basic Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University
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HAYASHI Masanobu
Department of Basic Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University
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ENDOH Daiji
Department of Veterinary Radiology, Faculty of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Japan
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