Analysis of the cell cycle of fibroblasts derived from the LEC rat after X-irradiation
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概要
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The LEC rat is reported to exhibit hypersensitivity to X-irradiation, deficiencyin DNA double-strand break repair, and radio-resistant DNA synthesis.This character of the LEC rat has been thought to be due to abnormal G１arrestin cells after X-irradiation. In this report, we re-investigated the effect ofX-irradiation on the cell cycle in primary-cultured fibroblasts . Primaryculturedfibroblasts derived from LEC and BN rats were exposed to４Gy of Xrayand their cell cycle analysis was performed with a flow cytometer. Fibroblastsderived from both rats showed normal response of the cell cycle, indicatingthe arrest at both G１‐and G２／M-phase and no difference in the cell cyclepopulation between fibroblasts derived from both rats. In contrast, when thesame analysis was performed using the cell line, L７and W８，which had beenestablished from the lung fibroblasts of LEC and control WKAH rats, respectively,by immortalizing with SV４０T-antigen, L７cells but not W８cells showedimpaired G１arrest and abnormal cell cycle. These results suggest that fibroblastsderived from LEC rats possess the normal cell cycle response after Xirradiation,if they are kept naive as not immortalized with SV４０T-antigen.
- The Graduate School of Veterinary Medicine, Hokkaido Universityの論文
- 2006-02-28
著者
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Tachi Atsushi
Laboratory Of Public Health Graduate School Of Veterinary Medicine Hokkaido University
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Agui T
Laboratory Of Laboratory Animal Science And Medicine Department Of Disease Control Graduate School O
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