Development and Application of West Nile Virus Subgenomic Replicon RNA Expressing Secreted Alkaline Phosphatase

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概要

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• We have developed a West Nile virus (WNV) subgenomic replicon harboring the secreted alkaline phosphatase (SEAP) reporter gene instead of viral structural genes (designated repWNV/SEAP). The repWNV/SEAP allowed easy evaluation of viral replication efficiency by direct measurement of SEAP secretion of the cell culture medium in physical containment level 2 facilities. Furthermore, we validated the availability of this system using the known anti-flavivirus gene, mouse oligoadenylate synthetase 1b (Oas1b). The Oas1b-transfected cells were more resistant to repWNV/SEAP replication than were the original cells. Thus, not only this system affords a useful tool for the identification/evaluation of anti-flavivirus genes/drugs in terms of safety, ease of use and reliability, but should be able to reduce or replace the bioassay using laboratory animals.

著者

• Maeda Akihiko

  Department Of Laboratory Animal Science Faculty Of Veterinary Medicine Hokkaido University

• Agui Takashi

  Laboratory Of Laboratory Animal Science And Medicine Department Of Disease Control Graduate School O

• SASAKI Nobuya

  Laboratory of Experimental Animal Science, Graduate School of Veterinary Medicine, Hokkaido Universi

• NISHINO Tomohiro

  Laboratory of Laboratory Animal Science and Medicine, Department of Disease Control, Graduate School

• NISHINO Tomohiro

  Laboratory of Laboratory Animal Science and Medicine, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University

• SASAKI Nobuya

  Laboratory of Laboratory Animal Science and Medicine, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University

• MORITOH Kanako

  Laboratory of Laboratory Animal Science and Medicine, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University

• MAEDA Akihiko

  Department of Animal Medical Sciences, Faculty of Life Science, Kyoto Sangyo University

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