The mRNA Regulation of Porcine Double-Stranded RNA-Activated Protein Kinase Gene
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概要
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The double-stranded RNA (dsRNA)-activated protein kinase (PKR), which is one of the products of interferon (IFN)-stimulated genes, participates in the biological actions of IFN such as antiviral effects and immune response. In the present study, we identified the primary structure of porcine PKR proteins by cDNA cloning. Porcine PKR protein consisted of 537 amino acids and had two dsRNA-binding domains similarly existing in PKR proteins of other species. The treatment with IFN-α induced the expression of <i>PKR</i> 3.9-fold in a porcine kidney cell line, LLC-PK1. The same results were obtained when the cells were treated with poly(I)·poly(C), but treatment with either IFN-γ or LPS did not induce this gene in LLC-PK1 cells. These results suggest similarity of the regulatory mechanisms in the <i>PKR</i> gene among mammalian species.<br>
著者
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Kon Yasuhiro
Laboratory Of Anatomy Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokka
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Asano Atsushi
Laboratory Of Biochemistry Department Of Biomedical Sciences Graduate School Of Veterinary Medicine
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Agui Takashi
Laboratory Of Laboratory Animal Science And Medicine Department Of Disease Control Graduate School O
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