Effects of inhibitors of nonselective cation channels on the acetylcholine-induced depolarization of circular smooth muscle from the guinea-pig stomach antrum
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概要
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In circular smooth muscle bundles isolated from the guinea-pig stomach antrum, the effects of quinidine, Ni^<2+>, flufenamic acid, niflumic acid, La^<3+>, SKF-96365 and 4'-diisothiocyanatostilbene-2, 2'-disulphonic acid (DIDS) on acetylcholine (ACh)-induced depolarization were investigated. Recording membrane potentials from smooth muscle cells with intracellular microelectrodes revealed that ACh (1μM) depolarized the membrane by 5-8 mV and increased the amplitude and frequency of slow potentials. These effects were inhibited by atropine. Quinidine (10μM) increased the amplitude of ACh-induced depolarization, with no alteration to the properties of slow potentials. Ni^<2+> (50μM) transiently (5-10 min) depolarized the membrane by about 5 mV, with an associated increase in frequency and amplitude of slow potentials. In the stabilized condition with Ni^<2+>, the amplitude of ACh-induced depolarization remained unchanged. Flufenamic acid (10μM) inhibited the generation of slow potentials, with no change in either the amplitude of ACh-induced depolarization or of the amplitude and frequency of slow potentials generated during ACh stimulation. A high concentration of flufenamic acid (100μM) depolarized the membrane and increased the amplitude of ACh-induced depolarization. Niflumic acid (10μM) hyperpolarized the membrane and increased the amplitude and frequency of slow potentials and also the amplitude of ACh-induced depolarization. DIDS (100μM) hyperpolarized the membrane and inhibited the amplitude and frequency of slow potentials, with no alteration to the amplitude of ACh-induced depolarization. SKF-96365 (3-50μM) depolarized the membrane in a concentration-dependent manner, but did not change the level of ACh-induced depolarization. La^<3+> (50μM) did not alter the properties of the slow potentials or the ACh-induced responses. These results provide evidence that ACh-induced depolarization is not inhibited by chemicals known to inhibit non-selective cation channels. We suggest that muscarinic receptor-mediated signal transduction may be different in smooth muscle and interstitial cells.
- 日本平滑筋学会の論文
著者
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Yokoyama Y
Department Of Physiology Nagoya City University School Of Nursing
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Yokoyama Yoshifumi
First Department Of Internal Medicine Nagoya City University School Of Medicine
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Kim Young
Department of Obstetrics and Gynecology, Women's Cancer Clinic, Yonsei University College of Medicin
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Kim Young
Department Of Electronics Engineering Korea Advance Intitute Of Science & Technology
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Yamamoto Y
Department Of Physiology Nagoya City University School Of Nursing
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YAMAMOTO Yoshimichi
Department of Physiology, Nagoya City University School of Nursing
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SUZUKI Hikaru
Department of Physiology, Nagoya City University Medical School
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HOTTA Aya
Department of Physiology, Nagoya City University Medical School
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NAKAMURA Eri
Department of Physiology, Nagoya City University Medical School
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KITO Yoshihiko
Department of Physiology, Nagoya City University Medical School
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Kim Young
Department Of Agricultural Chemistry The University Of Tokyo
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Yokoyama Yoshifumi
The First Department Of Internal Medicine Nagoya City University Medical School
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Kito Yoshihiko
Department Of Gastroenterology And Metabolism Nagoya City University Graduate School Of Medical Scie
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Hashimoto Ayako
Research Institute Of Pharmacological & Therapeutical Development Otsuka Pharmaceutical Co. Ltd.
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Suzuki H
Department Of Physiology Nagoya City University Medical School
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Suzuki Hikaru
Department Of Physiology Nagoya City University Medical School
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Suzuki Hikaru
Department Of Cell Physiology Nagoya City University Medical School
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Kim Young
Department Of Physiology Chungbuk National University College Of Medicine
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Yamamoto Yoshimichi
Laboratory Of Physiology Nagoya City University School Of Nursing
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Yamamoto Y
Department Of Physiology Nagoya City University Nursing School
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Yamamoto Yoshimichi
Department Of Civil Engineering
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Kamiya Yoshinobu
名古屋市立大学大学院医学研究科消化器・代謝内科学
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Kito Y
Department Of Gastroenterology And Metabolism Nagoya City University Graduate School Of Medical Scie
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Hotta Aya
Department Of Physiology Nagoya City University Medical School
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Hotta Aya
Department Of Biodefense Medicine Nagoya City University Graduate School Of Medical Sciences
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Suzuki Hikaru
Dep. Of Cell Physiology
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Nakamura Eri
Department Of Cell Physiology Nagoya City University Medical School
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Suzuki Hikaru
Department Of Cardiology Tokyo Women's Medical University
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