培養細胞におけるUbiquinone-9のとり込みに関する研究

概要

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Incorporation of ubiquinone-9 into cultured cells (FM3A) was studied using ^3H-labeled ubiquinone-9 (^*UQ-9) by two experimental procedures of microautoradiography and subcellular fractionation of the cells by sucrose density gradient. In microautoradiography, the exposure for 14 days was the best, when the cells were incubated for 30 min in the culture medium containing 1 or 0.5 μCi/ml of ^*UQ-9. In addition, the microautoradiograph showed uniform distribution of the grains, and not localization in the limited space of the cells. The obvious increase in the accumulation of ^*UQ-9 in the mitochondrial fraction was observed after 8 hr incubation, while the intracellular radioactivity was the highest in the supernatant fluid after 30 min, and successive incubation exclusively showed higher radioactivity in mitochondrial fraction.
公益社団法人日本薬学会の論文
1969-08-25