In Vivo Testing System for Determining the Estrogenic Activity of Endocrine-Disrupting Chemicals (EDCs) in Goldfish (Carassius auratus)

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概要

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• Vitellogenin (VTG) is believed to be an effective and sensitive biomarker to detect the effects of endocrine-disrupting chemicals (EDCs) on fish. An enzymelinked immunosorbent assay (ELISA) was developed in this study for the quantification of VTG in goldfish (Carassius auratus) blood plasma using a monoclonal antibody against carp lipovitellin which is known to cross-react with goldfish VTG, and a working range of 7.8 to 500 ng VTG/ml was established. A laboratory study involving the dosing of male goldfish for 28 days with a range of bisphenol-A (BPA) concentrations was conducted, and the established ELISA was used. There was no significant induction of VTG at the concentrations of 1 and 10 μg/l. Exposure to 100 μg/l and 1000 μg/l BPA for 28 days significantly elevated plasma VTG concentrations to 201 ± 90 μg/ml and 104552 ± 24920 μg/ml, respectively. To confirm the effect of temperature on VTG induction, male goldfish were exposed to the nominal concentration of 17β-es-tradiol (E2) 100 μg/l at 10° and 30°C for 10 days. Plasma VTG concentration in male goldfish increased more quickly in the fish exposed at 30°C than in those at 10°C. In addition, the plasma VTG concentration in the 30°C group (10463 ± 3268 μg/ml) was higher than that of the 10°C group (1.42 ± 1.6 μg/ml) after 1 day of exposure. Thus goldfish are useful for the investigation of EDCs under various conditions since they are relatively small in size, and the ELISA established in the present study will contribute to various types of research.

• 公益社団法人日本薬学会の論文
• 2001-04-01

著者

• TAKAO Yuji

  Faculty of Environmental Studies, Nagasaki University

• ARIZONO Koji

  Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto

• Kohra Shinya

  Faculty of Environmental Studies, Nagasaki University

• Tominaga Nobuaki

  Department of Chemical and Biological Engineering, Ariake National College of Technology

• 石橋 康弘

  Environmental And Symbiotic Sciences Prefectural University Of Kumamoto

• Mishima T

  Laboratory Of Fishery Nutritional Science Graduate School Of Marine Science And Engineering Nagasaki

• Kohra Shinya

  Faculty Of Environmental Studies Nagasaki University

• Kohra Shinya

  Faculty Of Liberal Arts Nagasaki University

• Arizono K

  Prefectural Univ. Kumamoto Kumamoto Jpn

• Arizono K

  Faculty Of Pharmaceutical Sciences Nagasaki University

• Arizono Koji

  Faculty Of Environment Studies Nagasaki University

• Soyano K

  Nagasaki Univ. Nagasaki

• Soyano Kiyoshi

  Institute For East China Sea Research Nagasaki University

• Soyano Kiyoshi

  Graduate School Of Science And Technology Nagasaki University

• Soyano Kiyoshi

  Graduate School Of Science And Technology

• Takao Y

  Faculty Of Environmental Studies Nagasaki University

• Takao Yuji

  Faculty Of Environmental Studies Nagasaki University

• 冨永 伸明

  Department Of Chemical And Biological Engineering Ariake National College Of Technology

• TACHIBANA Katsuyasu

  Graduate School of Marine Science and Engineering, Nagasaki University

• Nagae Masaki

  Faculty of Environmental Studies, Nagasaki University

• Ishibashi Yasuhiro

  Faculty of Human Environment, Nagasaki Institute of Applied Science

• Ishibashi Yasuhiro

  Environmental Protection Center, Nagasaki University

• Tsuchimoto Mutsuyosi

  Graduate School of Science and Technology, Nagasaki University

• Ishibashi Hiroshi

  Graduate School of Science and Technology, Nagasaki University

• Saito Koji

  Division Of Marine Biosciences Graduate School Of Fisheries Sciences Hokkaido University

• Tominaga Nobuaki

  Department Of Biochemistry School Of Pharmaceutical Sciences Nagasaki University

• Tsuchimoto Mitsuyosi

  Laboratory Of Fishery Nutritional Sciences Graduate School Of Marine Science And Engineering Nagasak

• Tsuchimoto M

  Nagasaki Univ. Nagasaki Jpn

• Tsuchimoto Mutsuyosi

  Graduate School Of Science And Technology Nagasaki University

• Tsuchimoto Mutsuyosi

  Laboratory Of Fishery Nutritional Sciences Graduate School Of Marine Science And Engineering Nagasak

• Nagae M

  Faculty Of Environmental Studies Nagasaki University

• Nagae Masaki

  Faculty Of Environmental Studies Nagasaki University

• Ishibashi Hiroshi

  Graduate School Of Science And Technology Nagasaki University

• Ishibashi Yasuhiro

  Faculty Of Human Environment Nagasaki Institute Of Applied Science

• Ishibashi Yasuhiro

  Environmental Protection Center Nagasaki University

• Tachibana Katsuyasu

  Graduate School Of Science And Technology Nagasaki University

• Tsuchimoto Mitsuyosi

  Laboratory Of Fishery Nutritional Sciences Graduate School Of Marine Science And Engineering Nagasak

• Tominaga Nobuaki

  Dep. Of Chemical And Biological Engineering Ariake National Coll. Of Technol.

• Kohra S

  Faculty Of Environmental Sciences Nagasaki University

• Ishibashi Yasuhiro

  Environmental Protection Center

• Takao Yuji

  Faculty of Environmental Sciences

• Kohra Shinya

  Faculty of Environmental Sciences

• Ishibashi Hiroshi

  Graduate School of Engineering, Hokkaido University

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