A Multi-Generation Sublethal Assay of Phenols Using the Nematode Caenorhabditis elegans

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概要

• 論文の詳細を見る

• The nematode Caenorhabditis elegans (C. elegans) was used for a multiple-generation toxicity bioassay of phenols. We examined the sublethal toxicity (fecundity and reproduction) of bisphenol A (BisA), 4-(1-adamantyl) phenol (Adp), and 4,4′-(1,3-adamantanediyl) diphenol (AdDP) over five generations using a Nematode Growth Medium (NGM) 1.7% agar plate. In the fourth generation, the phenols affected the fecundity rate of C. elegans at doses 100- to 10000-fold lower than the LC50s. In particular, at 1 nM BisA, the number of worms decreased significantly to about 50% of control (p < 0.05). A comparison of the number of viable worms and eggs suggested that the phenols exert hatching toxicity. In addition, individuals with an abnormality in the vulva, which could not ovulate, appeared on the phenol-containing plates. We confirmed that phenols disrupt reproduction in C. elegans and that the method using NGM agar plates facilitates multi-generational toxicity tests of chemicals that are poorly soluble in water.

• 公益社団法人日本薬学会の論文
• 2003-12-01

著者

• Kohra Shinya

  Faculty of Environmental Studies, Nagasaki University

• Tominaga Nobuaki

  Department of Chemical and Biological Engineering, Ariake National College of Technology

• Arizono Koji

  CREST, Japan Science and Technology Corporation

• Kohra Shinya

  Faculty Of Environmental Studies Nagasaki University

• Kohra Shinya

  Faculty Of Liberal Arts Nagasaki University

• Arizono K

  Prefectural Univ. Kumamoto Kumamoto Jpn

• Arizono K

  Faculty Of Pharmaceutical Sciences Nagasaki University

• Soyano K

  Nagasaki Univ. Nagasaki

• Soyano Kiyoshi

  Institute For East China Sea Research Nagasaki University

• Soyano Kiyoshi

  Graduate School Of Science And Technology

• 冨永 伸明

  Department Of Chemical And Biological Engineering Ariake National College Of Technology

• Saito Koji

  Division Of Marine Biosciences Graduate School Of Fisheries Sciences Hokkaido University

• Iguchi Taisen

  CREST, JST, Japan Science and Technology Corporation

• Tominaga Nobuaki

  Department Of Biochemistry School Of Pharmaceutical Sciences Nagasaki University

• Iguchi Taisen

  Crest Japan Science And Technology Agency:okazaki Institute For Integrative Bioscience National Inst

• Iguchi Taisen

  Crest Jst Japan Science And Technology Corporation:department Of Bio-environmental Research Center F

• Iguchi T

  Department Of Bio-enviromental Research Center For Integrative Bioscience National Institute For Bas

• Tominaga Nobuaki

  Dep. Of Chemical And Biological Engineering Ariake National Coll. Of Technol.

• Kohra S

  Faculty Of Environmental Sciences Nagasaki University

• Kohra Shinya

  Faculty of Environmental Sciences

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