Validation of an Enzyme-Linked Immunosorbent Assay Method for Vitellogenin in the Medaka
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概要
- 論文の詳細を見る
An enzyme-linked immunosorbent assay (ELISA) was validated for the measurement of vitellogenin (VTG) in the cyprinid model species, medaka (Oryzias latipes). Both polyclonal antibody- and monoclonal antibody-based kits showed very high correlation between VTG values in unknown samples when we used standardized VTG protein. This was confirmed by 5 laboratories which participated in the ring test. The use of one gold standard VTG is essential to measure VTG values using ELISA kits in future OECD ring tests to screen for estrogenic chemicals in the 3 fish species medaka, zebrafish and fathead minnow.
- 公益社団法人日本薬学会の論文
- 2004-06-01
著者
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鑪迫 典久
(独)国立環境研究所環境ホルモン・ダイオキシン研究プロジェクト淡水生物グループ
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Morita M
National Inst. Environmental Studies Tsukuba
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Morita Masatoshi
National Institute For Environmental Studies
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Morita M
Endocrine Disruptors And Dioxin Research Projects National Institute For Environmental Studies
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Iguchi Taisen
CREST, JST, Japan Science and Technology Corporation
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Iguchi Taisen
Crest Japan Science And Technology Agency:okazaki Institute For Integrative Bioscience National Inst
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Iguchi Taisen
Okazaki Institute For Integrative Bioscience National Institute For Basic Biology National Institute
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鑪迫 典久
(独)国立環境研究所
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鑪迫 典久
環境研 環境リスク研究セ
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鑪迫 典久
株式会社日本紙パルプ研究所
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鑪迫 典久
独立行政法人国立環境研究所環境ホルモン・ダイオキシン研究プロジェクト
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鑪迫 典久
(独)国立環境研究所化学環境研究領域生態化学研究室
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Iguchi T
Yokohama City Univ. Yokohama Jpn
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TATARAZAKO Norihisa
National Institute for Environmental Studies
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Morita M
Endocrine Disrupters Research Laboratory National Institute For Environmental Studies
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Hori Hiroyuki
Environmental Health And Safety Division Environmental Health Department Ministry Of The Environment
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Koshio Masaaki
National Institute for Environmental Studies
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鑪迫 典久
環境研
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Tatarazako N
National Institute For Environmental Studies
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