In Vivo Evaluation of 4-Methyl-2, 4-bis(4-hydroxyphenyl) pent-1-ene (MBP), a Bisphenol A Metabolite, Using the Nematode Caenorhabditis elegans(SPECIAL ISSUE CELEBRATING TEN YEARS OF ESTABLISHMENT OF FACULTY OF ENVIRONMENTAL STUDIES)

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In this study, we investigated the lethal and sublethal effects of 4-methyl-2 ,4-bis(4-hydroxyphenyl)-pent-l-ene (MBP), a bisphenol A (BPA) metabolite, using the nematode Caenorhabditis eleganas (C. elegans) grown on Nematode Growth Medium (NGM) 1.7% agar plates. The bioconcentration factor (BCF) was also determined. The 24 hr-median lethal concentration (24 hr-LC_<50>) value of MBP was 0.49 mM (134 mg/1), and the acute toxicity of MBP against C. elegans was slightly stronger than that of BPA. In contrast to BPA, the sublethal toxicity of MBP in a multi-generation test, as determined by reproduction and fecundity, was slightly lower. The growth of animals, as determined by body lenth, was enhanced by 3-8% at 0.1 mM MBP, after 48-72 hr. The BCF value after exposure for 48 hr was determined to be approximately 19-105 as compared with the nominal concentration.