High-Performance Liquid Chromatography of Fullerence (C_<60>) in Plasma Using Ultraviolet and Mass Spectrometric Detection

概要

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Fullerence (C_<60>) was determined by high-performance liquid chromatography using both ultraviolet and mass spectrometric detection. The detection limit for each method was 0.05 and 2.0ng (signal-to-noise ratio (S/N=2)) per injection, respectively. Rat plasma spiked with C_<60> (10μg/ml) was extracted using solid phase extraction with a recovery of 62.1% and the coefficient of variation (c.v., n=5) between intra-day assays was 4.0%. The calibration curve for peak area and plasma C_<60> concentration with ultraviolet detection showed good linearity (r=0.996) over the range 0.5-60 μg/ml. This newly developed method was applied to rat plasma samples after intravenous administration of C_<60> solubilized with polyvinylpyrrolidone.
社団法人日本薬学会の論文
1995-09-15